液泡型ATP酶C亚基ATP6V0C在人前列腺癌细胞系中的表达及其意义

摘要

目的 探讨液泡型ATP酶C亚基ATP6V0C在不同转移潜能人前列腺癌细胞系中的表达及其意义.方法 采用半定量RT-PCR、荧光实时定量RT-PCR和Western blot法检测ATP6V0C在人不同转移潜能前列腺癌细胞系PC-3M-1E8、PC-3M(高转移潜能)和PC-3M-2B4、PC-3(低转移潜能)中的表达.结果 ATP6V0C在PC-3M-1E8、PC-3M细胞系中的mRNA及蛋白表达量均明显高于在PC-3M-2B4、PC-3细胞系中的表达,其中,ATP6V0C在PC-3M-1E8中的表达最高,差异具有统计学意义(P<0.05).结论 ATP6V0C在高转移潜能人前列腺癌细胞系中的表达明显高于低转移潜能人前列腺癌细胞系,证明其和肿瘤的转移密切相关,有可能成为判断人前列腺癌侵袭和转移的重要指标及治疗前列腺癌的新靶点.%Purpose To investigate the expression and significance of C subunit of vacuolar ATPase ( ATP6V0C ) in human prostate cancer cell lines with different metastatic potential.Methods The expression of ATP6V0C was evaluated in different prostate cancer cell lines using RT-PCR, real-time RT-PCR and Western blot.Results The expression of ATP6V0C mRNA and protein in PC-3M-1E8 and PC-3M cell line with high metastatic potentiality was significantly higher than that in PC-3M-2B4 and PC-3 cell line with low metastatic potential.Of them all, the expression of ATP6V0C in PC-3M-1E8 was highest.Conclusion The expression of ATP6V0C in PC-3M-1E8 and PC-3M cell line with high metastatic potential is significantly higher than that in PC-3M-2B4 and PC-3 cell line with low metastatic potential, suggesting that ATP6V0C is closely related to prostate cancer metastasis.Therefore, ATP6V0C is an important prognostic indicator in judgement of prostate cancer cell growth, progression and metastasis, and targeting ATP6V0C may be a promising strategy against human prostate cancer.