目的 探讨脑缺血-再灌注损伤大鼠脑皮质微小核糖核酸(miRNAs)表达谱的变化情况.方法 取雄性SD大鼠12只,采用随机分组的方法将其分为脑缺血-再灌注(IR)组和假手术组,每组6只.采用线栓法建立大鼠大脑中动脉阻塞模型,缺血1.5 h,再灌注24 h.采用基因芯片分析技术观察IR后大鼠脑皮质中miRNAs表达谱的变化.利用生物信息学软件Targetscan及miRanda,预测与IR损伤相关的差异表达miRNAs的靶基因.结果 ①与假手术组比较,IR组大鼠脑皮质中有36个miRNAs异常表达>1.5倍(P＜0.05).其中9个miRNAs表达上调,包括rno-miR-27a、-32 、-99b 、-129、-129-2 、-153 、-207、-326和-494;27个miRNAs表达下调,包括rno-miR-195、-190、-146b、-20a、-301a、-434 、-411、-384-5p、-140、-191、-148b-3p、-9 、-23b、-320、-140 、-151、-106b、-29a 、-872、-434、-130a、-93、-487b、-185、-652、-382、-541.②Targetscan和miRanda预测数据库均有的与IR损伤相关的靶基因为:肿瘤坏死因子α诱导蛋白1(TNFAIP1)、肿瘤坏死因子受体超家族成员1A (TNFRSF1A)、补体C1q肿瘤坏死因子相关蛋白6(C1QTNF6)、白细胞介素10(IL-10)、白细胞介素6信号转导因子(IL6ST)、血小板源性生长因子β受体(PDGFRB)、水通道蛋白11(AQP11)、热休克蛋白90αA1( HSP90αA1)及 Bcl-2.结论 IR后大鼠脑皮质中miRNAs表达谱有明显变化.%Objective To investigate the changes of microRNA (miRNA) expression profiling in rat cerebral cortex during cerebral ischemia-reperfusion injury. Methods Twelve male SD rats were recrui-Led. They were randomly allocated into either an ischemia-reperfusion group or a sham operation group u-sing a completely randomized method (n = 6 in each group). A rat model of middle cerebral artery occlusion was induced by the intraluminal suture method with cerebral ischemia for 1. 5 hours and reperfusion for 24 hours. The changes of miRNA expression profiling in rat cortex after cerebral ischemia-reperfusion was observed by using microarray analysis technology. The cerebral ischemia-reperfusion injury related target genes of differential expression miRNAs were predicted using the bioinformatics software ( Targetscan and miRanda) . Results ①Compared to the sham operation group, 36 abnormal expressions were more than 1.5 times in the rat cortex of the ischemia-reperfusion group ( P ＜ 0. 05 ). The miRNA expressions in 9 of them were up-regulated, including rno-miR-27a, -32* , -99b* , -129, -129-2* , -153* , -207, -326, and -494; 27 miRNA expressions were down-regulated, including rno-miR -195, -190, -146b, -20a,-301a, -434* , -411, -384-5p, -140, -191, -148b-3p, -9* , -23b,- 320, -140* , -151, -106b, -29a* , -872, -434, -130a, -93, -487b, -185, -652, -382, and -541. ②Both Targetscan and Miranday prediction databases had the target base related to cerebral ischemia-eperfusion injury because of tumor necrosis factor alpha-induced protein l(TNFAIPl) , tumor necrosis factor receptor superfamily member 1A (TNFRSF1A) , complement-clq tumor necrosis factor-related protein 6 ( C1QTNF6) , interleukin 10 (IL-10) , interleukin 6 signal transducer ( IL6ST) , platelet-derived growth factor receptor β ( PDGFRB) , aquaporin 11 (AQP11) , heat shock protein 90αAl (HSP90αAl) , and Bel-2. Conclusion The microRNA expression profile in rat cortex changed significantly after cerebral ischemia-reperfusion.
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