Objective: To discuss the correlation between serum uric acid (SUA) and vascular endothelial function in rats with hyperuricemia (HUA). Methods: A total of 36 male SD rats were randomly and equally divided into three groups: normal control group, model group and allopurinol treatment group. To produce HUA model in rats, a high— yeast (YE) extract diet and intraperitoneal injection of oxonic acid potassium were used for six weeks. Allopurinol treatment group received modeling agent and allopurinol intragastrically for six weeks. Systolic blood pressure (SBP) was measured regularly. Rats were killed after six weeks and levels of SUA, nitric oxide (NO), endothelin— 1 (ET— 1) and SBP were measured. The expression of endotheliocyte nitric oxide synthetase (eNOS) in aortic tunica intima of SD rats was measured by immunohistochemical method. Results; Compared with normal control group, levels of SUA [(45. 1±5. 6) μmol/Lvs. (216. 0±6. 2) μmol/L, P＜0. 001], ET-1 [(85.4±8. 7) μg/L vs. (163. 1±7. 2) μg/ L] and SBP [ (115. 7±4. 1) mmHg vs. ( 156. 0±3. 8) ramHg)] significantly increased, levels of serum NO [ (24. 1 ±2. 0) μmol/L vs. (17. 2±3. 4) μmol/L] and expression of eNOS [ (48. 3±4. 2) vs. (38. 3±4. 5)] in aortic tunica intima significantly decreased in model group, P＜0. 05 all. Compared with model group, levels of SUA [ (44. 8±4. 3) μmol/L], ET—1 [ (92. 8±5. 0) μg/L] and SBP [ (119. 2±4. 3) mmHg] significantly decreased, level of serum NO [ (22. 1±2. 2) μmol/L] and expression of eNOS [ (46. 1±4. 2)] in aortic tunica intima significantly increased in allopurinol treatment group, P＜0. 05 all. There were no significant difference for above indexes between allopurinol treatment group and normal control group (P>0. 05). SUA level was positively correlated with levels of SBP and ET—1 (r = 0. 98, 0. 98, P＜0. 001 both) and negatively correlated with level of NO ( — 0. 70, P＜0. 001). Conclusion: There is a close relationship between hyperuricemia and vascular endothelial dysfunction in rats. Decrease of serum NO and increase of endothelin— 1 may be an important cause lead to hypertension. Allopurinol has a protective effect for vascular endothelial function.%目的:探讨高尿酸大鼠血尿酸与血管内皮功能的关系.方法:雄性SD大鼠36只,随机均分为正常对照组、模型组和别嘌醇治疗组.使用高酵母膏饲料联合氧嗪酸钾腹腔注射6周诱导大鼠高尿酸模型.别嘌醇治疗组除给予造模剂外同时以别嘌醇灌胃6周.定期测量大鼠收缩压.6周后处死大鼠,检测各组血清尿酸(SUA)、一氧化氮(NO)、内皮素-1(ET-1)、收缩压(SBP)等的变化,用免疫组化法检测大鼠主动脉内膜层内皮型一氧化氮合酶(eNOS)的表达量.结果:与正常对照组相比,模型组大鼠SUA水平[(45.1±5.6)μmol/L∶(216.0±6.2)μmol/L]显著升高(P＜0.001),ET-1[(85.4±8.7)μg/L∶(163.1±7.2)μg/L]、SBP[(115.7±4.1) mmHg∶(156.0±3.8)mmHg]显著升高,血NO[(24.1±2.0)μmol/L∶(17.2±3.4)μmol/L]及主动脉内膜层eNOS[(48.3±4.2)∶(38.3±4.5)]表达量显著降低(P均＜0.05);与模型组比较,别嘌醇组SUA[(44.8±4.3)μmol/L]、ET-1[(92.8±5.0)μg/L]、SBP[(119.2±4.3)mmHg]水平显著降低,血NO[(22.1±2.2)μmol/L]和主动脉内膜eNOS的表达量(46.1±4.2)明显升高(P均＜0.05),别嘌醇组与正常对照组比较上述指标无明显差异(P＞0.05).SUA与SBP、ET-1呈正相关(r=0.98、0.98,P均＜0.001),与NO呈负相关(-0.70,P＜0.001).结论:高尿酸血症与大鼠血管内皮功能紊乱密切相关.血一氧化氮的降低和内皮素-1的升高可能是发生高血压的重要原因之一.别嘌醇具有保护血管内皮功能的作用.
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