目的:研究不同类型冠心病(CHD)[稳定型心绞痛(SAP)、不稳定型心绞痛(UAP)],以及急性心肌梗塞(AMI)]患者血浆线粒体偶联因子-6(MCF-6)在冠脉循环中浓度的变化.方法:用放免法分别测定不同类型心绞痛的冠心病患者与正常对照组外周、冠状静脉窦与主动脉根部血浆中MCF-6浓度,计算心脏局部分泌的MCF-6浓度(冠状静脉窦-主动脉根部浓度的差值).结果:与正常对照组比较,CHD患者MCF-6浓度:外周[(294±40)pg/ml∶(265±40) pg/ml]、主动脉根部[(310±47) pg/ml∶(259±43) pg/ml]、冠状静脉窦[(384±42) pg/ml∶(271±37) pg/ml]、心脏局部分泌(74 pg/ml∶12pg/ml)的明显升高 (P均<0.01),冠状静脉窦MCF-6浓度明显高于主动脉根部及外周循环的(P均<0.01);且在CHD患者,外周、主动脉根部与冠状静脉窦血浆中MCF-6浓度随CHD严重程度增加而明显升高,以AMI组患者最高(P均<0.01),分别为[(358±51)pg/ml,(366±48)pg/ml,(420±56)pg/ml].结论:结果提示血浆线粒体偶联因子-6参与了冠心病的病理生理过程,在冠心病的发展过程中有可能是一种较为重要的血管活性物质.%Objective: To investigate changes of level of plasma mitochondrial coupling factor 6 (MCF- 6) in coronary circulation of patients with coronary heart disease (CHD) [ contain stable angina pectoris (SAP), unstable angina pec toris (UAP) and acute myocardial infarction (AMI)]. Methods: Radioimmunoassay was used to measure concentra tions of CF- 6 in peripheral circulation, aortic root (Ao) and coronary sinus (CS) of CHD patients and normal control group. The CS-Ao difference of MCF 6 was regarded as concentration of MCF- 6 secreted by local heart. Results:Concentrations of MCF- 6 in CHD patients were significantly higher than those in normal control group in peripheral circulation [ (294±40) pg/mlvs. (265±40) pg/ml], Ao [ (310±47) pg/mlvs. (259±43) pg/ml], CS[ (384±42) pg/mlvs. (271±37) pg/ml] andlocal heart (74 pg/mlvs. 12pg/ml), P＜0. 01all. The concentration of CF - 6 in CS was significantly higher than those in peripheral circulation and Ao (P＜0. 01 all). Among CHD patients, con centrations of plasma CF- 6 in peripheral circulation, Ao and CS increased along with the severity of CHD increased (P＜0. 01 all), and reached top in AMI patients [respectively were (358±51) pg/ml, (366±48) pg/ml, (420±56) pg/ml]. Conclusion: The results indicate plasma mitochondrial coupling factor - 6 may be involved in process of pathophysiology of coronary heart disease and may be one of important vasoactive agents in the development of coronary heart disease.
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