冠脉钙化危险因素与血清骨桥蛋白相关性分析

摘要

目的:探讨冠脉钙化(CAC)的独立危险因素,进一步分析血清骨桥蛋白(OPN)与CAC及其危险因素的相关性.方法:据64层螺旋CT冠脉造影结果连续入选65例患者,分为冠脉钙化(CAC)组(37例)和非冠脉钙化(UCAC)对照组(28例),用酶联免疫吸附法(ELISA)测定血清OPN水平.分别进行单因素和多因素Logistic回归分析研究冠脉钙化的危险因素,血清OPN与CAC危险因素的相关性采用Spearman's相关分析.结果:1、将单因素Logistic回归分析有统计学意义的年龄、高血压,糖尿病,饮食习惯不佳,缺乏运动,超重(OR=3.47～12.96,P=0.018～0.003)等变量引入多因素Logistic回归分析,结果多因素Logistic回归分析显示年龄、超重、睡眠质量差、饮食习惯不佳是CAC的独立危险因素,OR为35.31～5.17,P＜0.01～＜0.05;2、CAC组血清OPN水平显著高于UCAC组[(39.919±11.879) μg/L比(24.000±6.000) μg /L,P＜0.01];3、Spearman's 直线相关分析显示血清OPN水平与CAC危险因素:LDL-C、超重、年龄、TC呈正相关(r=0.487～0.286,P＜0.001～＜0.05),与睡眠质量差、糖尿病、不良饮食习惯、缺乏运动呈正相关(r=4.10～2.24,P＜0.01～＜0.05);与HDL-C呈负相关(r=-0.250,P＜0.05).结论:相关分析显示年龄、超重、睡眠质量差、不良饮食习惯等是CAC独立危险因素;血清OPN水平与LDL-C、超重、年龄、糖尿病、缺乏运动等相关.这说明应降低OPN水平,减少CAC危险因素,以减轻冠脉钙化、减慢其发展.%Objective:To explore independent risk factors of coronary artery calcification (CAC) and analyze correlation among risk factors of CAC and serum osteopontin (OPN) level.Methods:According to results of 64-slice spiral computed tomography (MSCT) coronary angiography,a total of 65 patients were continuously enrolled and divided into CAC group (n =37) and non-CAC control group (n =28).Enzyme linked immunosorbent assay (ELISA) was used to measure serum level of OPN.Single factor and multiple factor Logistic regression analysis's were used to analyze risk factors of CAC.Spearman's straight line correlation analysis was used to analyze correlation between risk factors of CAC and serum OPN.Results:1、The age,hypertension,diabetes,poor eating habits,lack of exercise,overweight,etc.,which were independent risk factors of CAC (OR =3.47～12.96,P =0.018～0.003) by single factor Logistic regression analysis,were inducted to multiple factor Logistic regression analysis,its result showed that age,overweight,poor sleep quality,poor eating habits were independent risk factors of CAC (OR =35.31～ 5.17,P＜0.01～＜0.05); 2、Serum level of OPN in CAC group was significant higher than that of non-CAC control group [ (39.919 ± 11.879) μg/L vs.(24.000 ± 6.000) μg /L,P＜0.01] ; 3、The Spearman straight line correlation analysis indicated that serum level of OPN was correlated with risk factors of CAC:positively correlated with LDL-C,overweight,age,TC ( r =0.487～0.286,P＜0.001 ～ ＜0.05),and positively correlated with poor sleep quality,diabetes,poor eating habits,lack of exercise ( r =4.10～2.24,P＜0.01 ～＜0.05) ;negatively correlated with HDL-C (r =-0.250,P＜0.05).Conclusion:Correlation analysis indicates that age,overweight,poor sleep quality,poor eating habits etc.are independent risk factors of CAC; Serum OPN level is correlated with LDL-C,overweight,age,diabetes,lack of exercise etc.,so these indicate that must decrease OPN level and risk factors of CAC to relieve CAC and slow down its development.