BCR-ABL融合抗原诱导特异性同源白血病融合细胞杀伤效应研究

摘要

Objective： The current study was designed to investigate the specific killing effects against homo-leukemia hybrid cell induced by BCR-ABL multiple epitope fusion protein and explore a powerful chronic myeloid leukemia(CML) immunotherapeutic strategy. Method： A multiple epitope antigen gene of BCR-ABL fusion antigen was used to immunize C57BL/6 mice and the spleen cells were cultured as effective cells. Specific lytic activities of target cells were detected by standard MTT assay. Results： Only the CTLs (cytotoxic lymphocytes) of mice immunized with the fusion protein can lyse the target cells but not the control cells (P<0.01 vs P>0.05). Conclusion： The multiple epitope BCR-ABL fusion antigen elicited antigen-specific, CTL-mediated anti-leukemia immune responses which provided a basis for further study on the developing specific T cell immunotherapy of CML in vivo.%目的：观察多表位BCR-ABL融合抗原在体外激发的免疫反应对同源白血病融合细胞的杀伤效应，探索慢性髓性白血病(chronicmyeloidleukemia,CML)免疫治疗的新途径。方法：用人工合成含有不同HLA(humanleukcyteantigen)限制性的BCR-ABL多个表位的融合蛋白免疫C57BL/6小鼠，取小鼠脾细胞进行体外培养，以四唑盐(MTT)比色法测定鼠CTLs(cytotoxiclymphocytes)介导的特异性杀伤效应。结果：融合抗原激发产生的鼠CTL能特异性杀伤靶细胞(P<0.01),但不杀伤对照组靶细胞(P>0.05)。结论：设计的多表位BCR-ABL融合抗原能激发特异性免疫反应，并对同源白血病细胞产生特异性杀伤，为进一步实验奠定了关键基础。