FRAGILE HISTIDINE TRIAD GENE EXPRESSION AND ITS CORRALATION WITH MISMATCH REPAIR PROTEIN IN HUMAN SPORADIC COLORECTAL CARCINOMA

摘要

Objective: To investigate the expression of fragilehistidine triad (FHIT) gene and its correlation withclinicopathological features and correlation with mismatchrepair protein (mainly MLH1 and MSH2) in humansporadic colorectal carcinoma (SCC). Methods:Immunohistochemistry SP method was used to determinethe expression of FHIT, MLH1 and MSH2 protein insurgically resected specimens of 84 human SCC. Results:The positive rates of FHIT, MLH1 and MSH2 proteinexpression were 48.81%, 92.86% and 100% respectively.Loss or reduced expression of FHIT protein was not related with tumors clinicopathological features such as age, gender, tumors site and histological type (P>0.05), but wascorrelated with tumors invade depth, degree of thedifferentiation, Ducks?stage and metastasis (P0.05) and MSH2 protein(r=0.0000, P=l.00) expression in human SCC. Conclusion:Absent or reduction of FHIT gene expression consists ofhigh proportion and is a frequent event in SCC. FHIT gene is involved in the development and progression of humanSCC and may be a candidate tumors suppressor gene. The relationship between alteration of FHIT gene expression and mismatch repair protein (mainly MLH1 and MSH2)deserved further study in human SCC.