Reduction of CAⅡ Expression in Gastric Cancer: Correlation with Invasion and Metastasis

摘要

Objective:Human carbonic anhydrases Ⅱ (CAⅡ) gene plays an important role in different cancer.However,its relevance to gastric cancer (GC) remains unclear.In the present study,we aimed to investigate the expression of CAⅡ in GC and explore its correlation with some clinicopathologic characteristics of GC.Methods:The expression of CAⅡ in 20 specimens of normal gastric mucosa,38 specimens of intraepithelial neoplasia and 112 specimens of gastric carcinoma were detected by immunohistochemical techniques.Survival in GC with CAⅡ expression was studied.Results:The positive rate of CAⅡ protein in normal gastric mucosa was significantly higher than that in intraepithelial neoplasia and gastric carcinoma (100％ vs.63.16％ and 28.57％,P＜0.001).The positive rate of CAⅡ protein was significantly higher in gastric carcinoma at early stages than that at advanced stages (70.0％ vs.19.57％,P＜0.001).The positive rate of CAⅡ protein was significantly lower in gastric carcinoma with lymph node metastases than that without lymph node metastases (10.81％ vs.37.33％,P＜0.05).Furthermore,the positive rate of CAⅡ protein was significantly lower in poorly-differentiated gastric carcinoma than in moderately-or well-differentiated gastric carcinoma (15.94％ vs.31.03％ or 60.00％,P＜0.05).Moreover,CAⅡ expression was not related with sex,age and tumor size.The patients with CAⅡ-positive tumors showed a better survival rate than those with CAⅡ-negative tumors (P=0.024,log-rank test).Conclusion:CAⅡ expression was related with stages and lymph node metastases in gastric carcinoma.The reduction of CAⅡ expression in GC might promote tumor cell motility and contribute to tumor growth and metastasis.