亚甲基蓝对猪凝血酶病毒灭活/去除工艺验证实验

摘要

Purpose To test the result of methylene blue virus inactivation/removal process on swine thrombin and to improve the production process. Methods Adding 0.5 mg/L methylene blue on Sindbis virus,of EMCV and PPV and PRV viruses,then putting them in the inactivated virus illumination instrument for 2 hours and using 60 kD ultrafiltration method to validate the feasibility of the process to inactivated virus. Results Methylene blue inactivated and filter virus could effectively remove the virus by ultrafiltration. Viruses which were incorporated into thrombin and in the original culture, lasty lose the ability to cell infection,and reduce the TCID50 higher than 4Log10. Conclusion Methylene blue virus inactivation/removal process can effectively inactivate the virus in swine thrombin.%目的 验证亚甲基蓝光化学法灭活猪凝血酶中病毒的灭活效果,完善生产工艺.方法 在SINV、EMCV、PPV和PRV等4种病毒中,加入0.5 mg/L的亚甲基蓝置于病毒光照灭活仪上光照2h和用60 kD膜超滤的灭活病毒方法来验证工艺的可行性.结果 经亚甲基蓝可有效灭活病毒,经超滤法滤除可有效去除病毒.掺入到凝血酶原液中的病毒以及在原培养物中病毒都丧失了对细胞的感染性,降低的滴度TCID50高于4 Log10.结论 亚甲基蓝光化学法可有效灭活猪凝血酶中病毒.