人参皂苷纳米乳的美白抗衰作用及其安全性评价

摘要

目的：研究人参皂苷纳米乳的美白抗衰作用及其安全性。方法美白作用：用维生素C为对照药品，测定酪氨酸酶抑制率。抗衰老作用：建立D-半乳糖大鼠衰老模型，在大鼠颈背部皮肤上涂抹人参皂苷纳米乳及对照药品，每日2次，制备涂片，比较皮肤组织纤维的排列变化，测定DPPH自由基清除率。安全性评价：观察皮肤涂抹人参皂苷纳米乳及对照药品后，不同时间的皮肤变化情况。结果人参皂苷浓度为7.00 mg／mL时，酪氨酸酶抑制率顺序是，维生素C水溶液＞人参皂苷纳米乳＞人参皂苷水溶液；在衰老大鼠皮肤上涂抹人参皂苷纳米乳，切片结果显示，弹力纤维与皮肤表面较为平行，而未涂抹的对照组，弹力纤维排列较为曲折，与皮肤表面不平行；DPPH自由基清除率结果显示，维生素C水溶液＞人参皂苷纳米乳＞人参皂苷水溶液，且随着人参皂苷载药量的增大，人参皂苷纳米乳清除DPPH自由基的效率明显上升；安全性结果显示，皮肤涂抹人参皂苷纳米乳后，没有红肿、刺激等现象发生。结论人参皂苷纳米乳比人参皂苷水溶液具有更明显的美白抗衰作用，没有刺激，不会对皮肤造成损伤，安全可靠。%Objective To study on whitening and anti -aging effect of ginseng saponin and its safety.Methods Whitening effect:using vitamin C as the control drug, the inhibition rate of tyrosinase was determined.Anti senescence effect: the aging model of D-was established, and the DPPH was applied to the skin of the rat's neck, and the drug was prepared by 2 times a day.Skin safety evaluation: the skin changes of the skin of the ginseng saponins were observed after the skin was given ginseng saponin and control drugs.Results When the concentration of ginsenoside was 7 mg/mL, the inhibition rate order was, the water solution of vitamin C >ginsenoside nano >ginsenoside aqueous solution ; In the aging model, the surface of the elastic fibers and the skin surface was parallel to the skin, but the elastic fibers were arranged in a more tortuous and non parallel to the skin;DPPH free radical scavenging effect order was, Vitamin C aqueous solution>ginsenoside nano>ginsenoside aqueous solution, and with the dosage of ginsenoside increasing, the efficiency of ginsenoside DPPH scavenging free radical increased significantly;Skin safety evaluation results showed that ginseng saponin nanometer milk smeared skin, no redness, irritation and other phenomena occured.Conclusion Ginseng saponin nanometer milk has obvious whitening effect, and can not cause damage to the skin, is safe and reliable.