流感病毒对小鼠脾淋巴细胞增殖的影响及板蓝根的干预作用

摘要

Objective: To investigate the response of lymphocyte proliferation in spleen of the BALB/c mice with influenza virus(H1N1 ) , and therapeutic effect of Chinese Medicine. Methods; FM1 virus(H1N1) was dropped nasally in BALB/c mice with oral administration of Banlangen granule and Ribavirin granule respectively. Status of mice, weight and lymphocyte proliferation of spleen for every group were observed before and after treatment. Results: Compared with that in model group, weight of spleen was kept more in normal group( P < 0.01) , and that in Ribavirin granule group was lost more ( P < 0.01) . Lymphocyte proliferation in Banlangen group and Ribavirin group with Con A was increased significantly ( P < 0.01, P < 0.05 respectively) which being compared with that in model group. Conclusion: In acute infection phase with the influenza virus(H1N1) .weight of spleen of mice was lost,lymphocyte proliferation response in Banlangen and Ribavirin groups with Con A can be enhanced, especially in Banlangen group.%目的:观察流感病毒感染后小鼠脾淋巴细胞增殖的变化,以及板蓝根颗粒的干预作用特征.方法:采用流感病毒(FM1)滴鼻感染BALB/c小鼠模型,以板蓝根颗粒进行干预,以利巴韦林为阳性对照药,分别灌胃给药.观察感染及用药前后各组小鼠的状态、脾脏重量,以及脾淋巴细胞增殖反应的变化.结果:与正常组相比,模型组脾脏重量减轻,差异显著(P<0.01);利巴韦林组脾重低于模型组(P<0.01).脾淋巴细胞增殖反应:经ConA诱导后,与模型组相比,利巴韦林组、板蓝根组淋巴细胞增殖反应均升高,差异显著(分别为P<0.05,P<0.01),其中板蓝根组升高趋势更明显.结论:流感病毒感染小鼠急性期脾脏重量减轻,脾淋巴细胞增殖反应受到抑制;板蓝根颗粒和利巴韦林颗粒具有促进流感病毒感染小鼠脾淋巴细胞的增殖作用,板蓝根颗粒的作用可能更明显.