Objective To investigate the changes and clinical significance of Caveolin-1,matrix metalloproteinase-9 (MMP-9) and interleukin-1β (IL-1β) in cerebrospinal fluid of children with bacterial meningitis or viral encephalitis.Methods Thirty-six cases of children with bacterial meningitis,42 cases of children with viral encephalitis,and 20 cases of children with non-nervous system infection were selected from September 2016 to June 2018 at the Third Affiliated Hospital of Zhengzhou University.The levels of Caveolin-1,MMP-9 and IL-1β in cerebrospinal fluid were detected by using enzyme linked immunosorbent assay (ELISA).Results Cerebrospinal fluid Caveolin-1,MMP-9,IL-1β levels in the acute phase of bacterial meningitis were(49.06 ± 8.96) ng/L,(134.79 18.88)μg/L,(100.02 ± 14.67) μg/L,respectively,and (29.13 ± 7.25) ng/L,(18.69 ± 7.23) μg/L,(47.57 ± 8.95)pg/L in recovery phase,which were higher than those of the controls [(11.18 ± 2.24) ng/L,(11.53 ± 3.54) μg/L,(39.75 ± 7.08) μg/L)],and the differences were significant (all P ＜ 0.05).Cerebrospinal fluid Caveolin-1,MMP-9,IL-1β levels in the acute phase of viral encephalitis were (42.71 ± 10.48) ng/L,(62.78 ± 17.39) μg/L,(57.97 ± 11.28) μg/L,respectively,and (29.13 ± 7.25) ng/L,(18.69 ± 7.23) μg/L,(47.57 ± 8.95) μg/L in recovery phase,which were higher than those of controls,and the differences were significant (all P ＜ 0.05).The levels of Caveolin-1,MMP-9 and IL-1β in cerebrospinal fluid of bacterial meningitis group and viral encephalitis group were significantly higher than those of convalescent group (all P ＜ 0.05).The levels of Caveolin-1,MMP-9,IL-1β in cerebrospinal fluid of bacterial meningitis group were significantly higher than those in viral encephalitis group (all P ＜ 0.05) in the acute phase,and no significant difference was found in the recovery phase(all P ＞ 0.05).Cerebrospinal fluid Caveolin-1,MMP-9,IL-1β showed no significant difference among children with different severity of intracranial infection.Correlation analysis showed that there was a positive correlation between Caveolin-1,MMP-9 and IL-1 β levels in cerebrospinal fluid of acute in bacterial meningitis group and viral encephalitis group (Caveolin-1 and MMP-9:R2 =0.239,P ＜ 0.05;MMP-9 and IL-1β:R2 =0.766,P ＜0.01;Caveolin-1 and IL-1β:R2 =0.245,P ＜ 0.05).Conclusions Caveolin-1,MMP-9 and IL-1 β involved in the pathogenesis of intracranial infection in children,and the effects of different pathogens on intracranial infection were different.%目的 探索细菌性脑膜炎及病毒性脑炎患儿不同时期脑脊液陷窝蛋白-1(Caveolin-1)、基质金属蛋白酶-9 (MMP-9)及白细胞介素-1β(IL-1β)水平变化的意义.方法 选取2016年9月至2018年6月在郑州大学第三附属医院住院的细菌性脑膜炎患儿36例,病毒性脑炎患儿42例,同期住院的非颅内感染患儿20例为对照组.采用酶联免疫吸附试验(ELISA)检测3组患儿脑脊液Caveolin-1、MMP-9及IL-1β水平.结果 细菌性脑膜炎组急性期脑脊液Caveolin-1、MMP-9及IL-1β的水平分别为(49.06±8.96) ng/L、(134.79 ±18.88)μ,g/L、(100.02±14.67) μg/L,在恢复期分别为(32.99±9.41) ng/L、(21.61±4.36) μg/L、(52.03 ±9.99)μ,g/L,均高于对照组[(11.18±2.24) ng/L、(11.53 ±3.54) μg/L、(39.75±7.08) μg/L],差异均有统计学意义(均P＜0.05).病毒性脑炎组急性期脑脊液Caveolin-1、MMP-9及IL-1β的水平分别为(42.71±10.48)ng/L、(62.78±17.39) μg/L、(57.97±11.28) μg/L,恢复期分别为(29.13 ±7.25) ng/L、(18.69±7.23)μg/L、(47.57±8.95) μg/L,均高于对照组,差异均有统计学意义(均P＜0.05).细菌性脑膜炎组及病毒性脑炎组急性期脑脊液Caveolin-1、MMP-9及IL-1β的水平均高于恢复期,差异均有统计学意义(均P＜0.05).细菌性脑膜炎组急性期脑脊液Caveolin-1、MMP-9及IL-1β水平均高于病毒性脑炎组,差异均有统计学意义(均P ＜0.05);2组恢复期脑脊液Caveolin-1、MMP-9及IL-1β水平比较,差异均无统计学意义(均P＞0.05).颅内感染患儿轻症组、重症组脑脊液Caveolin-1、MMP-9及IL-1β的水平无明显统计学差异(P＞0.05).相关性分析表明,急性期细菌性脑膜炎组、病毒性脑炎组脑脊液中Caveolin-1、MMP-9及IL-1β水平呈正相关(Caveolin-1与MMP-9:R2=0.239,P＜0.05;MMP-9与IL-1β:R2=0.766,P＜0.01;Caveolin-1与IL-1β:R2=0.245,P＜0.05).结论 Caveolin-1、MMP-9及IL-1β参与颅内感染的发病机制,且在不同病原导致的颅内感染中的作用不同.
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