气相色谱-三重四极杆质谱分析人参炮制品中的挥发性成分

摘要

The research of chemical changes in ginseng processing has been focusing on saponins and sugars.In this paper, volatile components in ginseng were studied for the first time, which provide a substance basis for different processed ginseng.The volatile components and their derivative paths of fresh ginseng, sun-dry ginseng and red ginseng were studied by gas chromatography-mass spectrometry(GC-MS/MS).The volatile components were detected and analyzed using TG-5SILMS nonpolar GC column, with He as the carrier gas through the NIST MS Spectral Database.Thirty, thirty-three and thirty-four species of volatile compounds in fresh ginseng, sun-dry ginseng and red ginseng were detected, respectively.The content of (-)-spartak alcohol in sun-dry ginseng is 31.98 times higher than that in the fresh ginseng, while the levels of eight volatile components such as octanal in sun-dry ginseng is three times higher than those in the fresh ginseng.The contents of ten volatile components such as cyclodecanone in red ginseng are 3 times higher than those in the fresh ginseng.Four volatile compounds in sun-dry ginseng and in red ginseng are not detected in fresh ginseng.%人参炮制的化学成分变化研究主要集中在皂苷和糖类,本文首次从挥发性成分角度阐释了人参不同炮制品的物质基础. 利用气相色谱-质谱联用(GC-MS/MS)方法,对鲜参、生晒参和红参中挥发性成分及其衍生规律进行研究. 采用TG-5SILMS非极性气相色谱柱,以He为载气,通过NIST MS Spectral Database对挥发性成分进行检测并鉴定. 鲜参、生晒参、红参中分别检出30、33和34种挥发性成分,其中生晒参中(-)-斯巴醇含量为鲜参含量的31.98倍,辛醛等8种挥发性成分为鲜参中含量的3倍以上,红参中有环癸等10种挥发性成分为鲜参中含量的3倍以上. 生晒参和红参中各有4种挥发性成分在鲜参中未检出.