生物纳米孔分析技术研究进展

摘要

生物纳米孔传感技术因其快速、低成本、无需荧光标记等优点,在化学和生物等诸多研究领域得到广泛应用,已发展成为一种新颖的、独具特色的单分子分析手段. 该技术目前主要应用于DNA测序研究,同时在单分子分析领域也取得了令人瞩目的成就. 该文简要介绍了生物纳米孔分析技术的原理和生物孔的种类,主要总结了近20年来生物纳米孔在DNA测序和单分子分析中的研究进展并予以了展望.%Biological nanopore sensing has been developed as an attractive single-molecule tool with advantages of being rapid, low-cost, and label-free.Nanopores are not only being widely developed for DNA sequencing, but they also have been utilized to analyze a wide range of analytes at the single-molecule level.This review summarizes and prospects the principle of the nanopore analysis and nanpore category, with an emphasis on the progress in nanopore-based DNA sequencing and single-molecule analysis.