基于高密度SNP芯片技术对中国西门塔尔牛SCD1基因与肉质性状的相关性分析

摘要

试验借助高密度SNP芯片技术在整个基因组范围内对223头中国西门塔尔牛群体进行基因分型,在SCD1基因上发现7个SNPs位点,对各SNP位点与西门塔尔牛肉质性状进行相关性分析.结果表明,SCD1基因的7个SNPs位点均与大理石花纹等级显著相关(P＜0.05或P＜0.01)；A10050C、A13655G、C14790T和A15565G 4个位点对剪切力影响显著(P＜0.05)；位点A13655G对肌内脂肪含量影响显著(P＜0.05)；位点A10050C、C14790T、A15565G不同基因型个体间脂肪颜色差异显著(P＜0.05).单体型分析结果显示,7个SNPs位点共构成6种单体型和14种单体型组合.单体型组合H3H6与高肌内脂肪含量极显著相关(P＜0.01)；单体型组合H6H6与优质大理石花纹极显著相关(P＜0.01)；单体型组合H4H6与高剪切力值显著相关(P＜0.05)；各单体型组合对肉色和脂肪颜色影响不显著.%SNPs of the whole genome were genotyped through high-density SNP chip technology in a population with 223 Chinese Simmental. Seven SNPs were found in SCD1 gene. The results of association of the seven loci with meat quality traits showed that the loci were all significantly correlated with marbling score(P<0. 05 or P<0. 01). The effect of different genotypes at A10050C, A13655G, C14790T and A15565G on sheare force were significant (P<0. 05). The locus A13655G was correlated with intramuscular fat(IMF) content, and individuals with AArn genotype has higher IMF (P < 0. 05). The effect of different genotypes at locus A10050C, C14790T and A15565G on fat color were significantly different(P<0. 05). The result of haplo-type analysis showed that 6 haplotypes and 14 haplotype combinations were detected. For IMF content, the individuals with haplotype combination H3H6 was significant higher than others at 0. 01 level while the individuals with haplotype combination H6H6 had a higher marbling score and the individuals with haplotype combination H4H6 had a higher sheare force. The effect of the haplotype combinations on meat color and fat color was not significant.