PAX3转录因子在羊驼皮肤组织中的表达和定位分析

摘要

本研究旨在研究PAX3在羊驼皮肤组织中的表达和分布.利用免疫组织化学和Western blot技术研究PAX3转录因子在不同被毛颜色羊驼皮肤组织中的表达与分布.结果表明PAX3在毛球基底层细胞之间,毛乳头周围和毛根部都存在阳性表达,并在不同被毛颜色羊驼皮肤组织中的表达存在显著差异(P＜0.05).结果提示PAX3参与了黑色素细胞的增殖、分化和迁移,PAX3与色素的形成相关.%This experiment was conducted to study PAX3 expression and location in alpaca skin. In the study, the distribution of PAX3 protein was studied by immunohistochemistry,the expression level was analyzed by Image-Pro Plus software and Western blot. The result showed PAX3 protein was mainly located in melancytes during the basal cells of hair bulb, around dermis papilla and in hair root. By Image-Pro Plus software and Western blot analysis, the protein expression level was significantly different in different coat color (P<0. 05). Based on the result, it indicates that PAX3 plays an important role in the melanocytes proliferation, differentiation and pigmentation.