如皋鸡不同时期肌肉组织生长相关基因的表达谱分析

摘要

The objective of this experiment was to study the development mechanism of muscle in Rugao chicken. The gene expression of Rugao chicken in muscle was analyzed during the different periods (2-12 week old) by GeneChip. The expression profiles of 13 379 differentially expressed genes were obtained, and the great changes of gene expression in muscle took place at chicken different growth stages. Compared with 2 week old, the number of down-regulated genes at 4 and 12 week old were more than that of up-regulated genes, while the number of down-regulated genes at 6, 8 and 10 week old were less than that of up-regulated genes. The clustering analysis of hybridization data was also carried out to indicate gene expression at different growth stages. The differential expressions of four genes were confirmed by real-time quantitative PCR, and the results indicated that the expression changes of these genes were generally consistent with theresults of GeneChip. Expression profiles of different growth times were constructed in order to establish dynamic expression profiles, by which the responsive genes affecting muscle growtn were entirely acquired, and the growth and development mechanisms of. Muscle at different growth stages were also revealed in Rugao chicken.%旨在了解如皋鸡不同时期肌肉生长发育机制.本研究利用基因芯片技术,分析在不同生长阶段下(2～12周龄)如皋鸡肌肉组织基因的表达情况.获得了13 379个基因的差异表达动态图谱.在不同生长阶段下肌肉组织生长基因的表达发生变化,与2周龄相比,4和12周龄下调表达的基因数量多于上调表达的基因数量,6、8和10周龄下调表达的基因数量少于上调表达的基因数量.同时对杂交数据进行多种聚类分析.利用实时荧光定量PCR验证4个基因的差异表达,其结果和芯片杂交分析的结果基本一致.初步建立了不同时期肌肉生长应答基因的动态表达谱,比较全面地获得了肌肉生长应答基因,初步揭示了如皋鸡不同时期肌肉生长发育机制.