番鸭呼肠孤病毒人工感染雏番鸭肝的蛋白质组学分析

摘要

应用蛋白质组学技术研究人工感染番鸭呼肠孤病毒(MDRV)的番鸭肝和健康对照番鸭肝的蛋白质组差异,为深入研究MDRV的致病机制奠定理论基础.通过二维双向凝胶电泳技术和MALDI-TOF-TOF质谱仪分析获取MDRV感染组与健康对照组之间的差异表达蛋白质,并运用蛋白质搜索鉴定软件Mascot 2.3.02鉴定蛋白质.结果共鉴定出38个差异表达蛋白质.比较感染组和健康对照组结果,感染组番鸭肝中有10个表达下调的蛋白质和17个表达上调的蛋白质,7个表达蛋白质仅出现在健康对照组,4个表达蛋白质仅出现在感染组.MDRV感染组的番鸭肝有肝细胞、神经细胞和肌肉细胞受损现象,同时存在细胞修复机制.差异蛋白质组学为MDRV分子发病机制的分析提供了一个新途径.%The purpose of this paper was to study the pathogenesis of Muscovy duck reovirus (MDRV) infected muscovy ducking.Forty-four healthy muscovy ducklings were divided into treatment group challenged with MDRV and control one injected with Hank's.Livers were collected at the 5th day after challenge.Proteomics of the livers were determined by differential proteomic technology.Differential protein spots between both groups were performed and analyzed by two-dimensional gel electrophoresis and MALDI-TOF-TOF mass spectrometer,and then they were identified by protein search software,Mascot 2.3.02.Thirty-eight differential protein spots were separated and identified.Ten down-regulated protein spots and seventeen up-regulated protein spots were observed in the liver samples of the treatment after comparison between the two groups.Seven protein spots were appeared only in the livers of control and four protein spots were only emerged in the livers of treatment.The results indicate that differential proteomics in livers,which were impacted and damaged by MDRV may provide a new idea of pathogenesis of MDRV infected muscovy duckling.