猪瘟病毒通过网格蛋白介导的内吞途径入侵ST细胞

摘要

病毒入侵易感细胞是病毒建立感染的必要过程,猪瘟病毒如何入侵易感细胞尚未明确.笔者对猪瘟病毒入侵细胞与网格蛋白介导的内吞途径的关系进行了初步研究.通过利用抑制剂氯丙嗪和Dynasore及shRNA技术,对网格蛋白及动力蛋白功能进行抑制,干扰网格蛋白介导的内吞途径,发现猪瘟病毒的细胞入侵效率明显下降;通过利用内体酸化抑制剂NH4Cl及shRNA技术下调内体标志蛋白Rab5和Rab7的表达量,发现内体酸化过程受到抑制后猪瘟病毒的感染效率受到了明显抑制.本研究初步证明猪瘟病毒能够利用网格蛋白介导的内吞途径完成对易感细胞的入侵,感染的过程依赖于初级内体和次级内体,为了解猪瘟病毒的感染过程积累了新的数据.%Cell entry of virus is an indispensable process in virus life cycle.However,the mechanism of classical swine fever virus (CSFV) cell entry is still undiscovered.Here,CSFV entry into ST cells by clathrin-mediated endocytosis was tested.Chemical inhibitor chlorpromazine and Dynasore and shRNA were used to disturb the clathrin-mediated endocytosis.The cell entry efficiency of CSFV was greatly decline when the function of clathrin and dynamin-2 were blocked.By using NH4Cl and down-regulation of Rab5 and Rab7 gene,CSFV infection was inhibited.These results showed that the CSFV can entry into susceptible cells by using clathrin-mediated endocytosis pathway and the early endosome and late endosome were used for CSFV infection.This research provides new information to understand the CSFV infection.