FGF7亚家族在小鼠毛囊第一生长周期的表达

摘要

旨在研究FGF7亚家族(FGF7、FGF10和FGF22)在小鼠毛囊第一生长周期中各阶段的表达情况,阐明其表达规律.取出生后第1、3、5、8、16、18、20和23天正常小鼠背部皮肤各3份,采用实时荧光定量PCR、Western blot和免疫组织化学技术检测FGF7、FGF10和FGF22蛋白及基因的表达.结果表明:FGF7主要在毛囊内根鞘及表皮表达;FGF10和FGF22广泛表达于毛囊各部.FGF7在毛囊退化期(16 d)表达量最低,静止期(18 d)和生长期(1～8、20～23 d)的表达量显著(P＜0.05)或极显著(P＜0.01)高于退化期(16 d);FGF10在毛囊生长期末期(8 d)表达量最低,生长期(1～5、20～23 d)、退化期(16 d)和静止期(18 d)表达量显著(P＜0.05)或极显著(P＜0.01)高于生长期末期(8 d);FGF22在毛囊退化期(16 d)表达量最高,静止期(18 d)和生长期(1～8、20～23 d)的表达量显著(P＜0.05)或极显著(P＜0.01)低于退化期(16 d).综上表明,FGF7、FGF10在毛囊第一生长周期可能对诱导毛囊进入新的循环周期有重要作用,而FGF22则可能对诱导毛囊进入退化期有重要影响.%The aim of this study was to investigate the expression patterns of fibroblast growth factor (FGF) 7 subfamily (FGF7,FGF10 and FGF22) in the first hair follicle growth cycle of mice.The Real-time PCR,Western blot and immunohistochemistry were adopted to study the expression of FGF7/FGF10/FGF22 mRNA and protein in mice back skin on 1,3,5,8,16,18,20,23 postnatal days.The results of immunohistochemistry showed that FGF7 protein was mainly located in the inner root sheath and epidermal;FGF10 and FGF22 were widely located in various structure of hair follicle.The results of real-time PCR,Western blot as well as immunohistochemistry showed that the FGF7 level was the lowest in catagen(16 d).And relative level of FGF7 was significantly increased in telogen (18 d) and anagen (1-8,20-23 d),compared to its expression in catagen (16 d) (P＜0.05,P＜0.01).The FGF10 level was the lowest in the end of anagen (8 d).And the relative level of FGF10 was significantly increased in prophase and metaphase of anagen (1-5,20-23 d),catagen (16 d) and telogen (18 d) compared to its expression in the end of anagen (8 d) (P＜0.05,P＜0.01).The FGF22 level was the highest in catagen (16 d).And relative level of FGF22 was significantly decreased in telogen (18 d) and anagen (1-8,20-23 d) compared to its expression in the catagen (16 d) (P＜0.05;P＜0.01).These results suggested that FGF7 and FGF10 might play an important role in inducing hair follicle into the new cycle during the development of hair follicle,and FGF22 might have an important effect on the induction of hair follicles into catagen.