This paper was aimed to study the effects of glucose oxidase on average daily gain and intestinal flo-ra in mice with moldy feed. A total of 130 KM mice, 18 to 22 g,were randomly assigned to 13 groups. The diet was added by 0, 10%, 20% and 30% moldy maize flour and 0, 0.1%, 0.3% and 0.5% GOD, respec-tively, and the experiment lasted for 60 days. The results showed as follows:compared with experimental con-trol group, the average daily gain of mice fed with the diet added by moldy maize flour and 0.3% GOD for 60 days was significantly increased ( P<0.05 or P<0.01) . Addition of 0.3% and 0.5% GOD to the diet with 10%moldy maize flour significantly decreased the amount of bacterium E. coli (P<0.01), and increased the a-mount of Laetobacillus ( P<0.01) . When the mice were fed for 60 days with the diet added 10% moldy maize flour, GOD significantly decreased the amount of bacterium E. coli, and increased the amount of Laetobacillus ( P<0.01) . GOD decreased the amount of bacterium E. coli in mice fed with 30% moldy maize flour diet ( P<0.05 or P<0.01) , and 0.3% GOD was proper. Addition of 0.3% and 0.5% GOD increased the amount of La-etobacillus in mice fed for 60 days ( P<0.01) . This study demonstrates that GOD can ameliorate the effects of moldy diet on the growth of mice. Addition of 0.3% GOD is suggested on the condition that aflatoxin B1 dose in diets is not more than 67.77 μg/kg.%本试验旨在研究葡萄糖氧化酶（ GOD）缓解霉变饲料对小鼠平均日增重和肠道菌群变化的影响。18～22 g昆明小鼠130只，随机分为13组。分别饲喂添加0、10％、20％、30％霉变玉米面和0、0．1％、0．3％、0．5％ GOD的饲料，试验期为60 d。结果表明：1）不同霉变玉米添加量的饲料饲喂小鼠60 d，与试验对照组比较，0．3％ GOD能显著或极显著改善小鼠平均日增重（ P＜0．05或P＜0．01）。2）添加10％霉变玉米面，0．3％和0．5％ GOD组与试验对照组比较，极显著降低了大肠杆菌的数量（P＜0．01），增加了乳酸杆菌数量（P＜0．01）；添加20％霉变玉米面，饲喂60 d时，3种不同浓度的GOD极显著降低了大肠杆菌数量（ P＜0．01），提高了乳酸杆菌数量（ P＜0．01）；添加30％霉变玉米面，3种不同浓度的GOD显著或极显著减少了肠道大肠杆菌数量（ P＜0．05或P＜0．01），0．3％ GOD效果较好，饲喂60 d时，0．3％和0．5％的GOD添加量极显著提高了肠道乳酸杆菌数量（ P＜0．01）。结果提示，GOD能够缓解霉变饲料对小鼠生长造成的影响。饲料中黄曲霉毒素B1含量低于67．77μg/kg时，添加0．3％的GOD效果较好。
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