In order to investigate the effects of diets added different levels of Perilla frutescens seed extracts on performance, reproductive hormone and immune function of the laying hens during the late laying peak period, a total of four hundred of eighty 48?week?old Hyline brown laying hens with the similar body weight and laying rate were randomly divided into 4 groups with 6 replicates per group and 20 hens per replicate in a single?factor completely randomized experimental design. Control group was fed a basal diet, the experimental groupsⅠ,Ⅱand Ⅲ were fed the basal diet added with 0.01%, 0.02% and 0.03% Perilla frutescens seed extracts, respec?tively. The experiment lasted for 30 days. The results showed as follows:1) compared with the control group, the diets added 0.01%, 0.02% and 0.03% Perilla frutescens seed extracts reduced the ratio of feed to egg of laying hens, but there was no significant difference ( P>0.05) . Among the 4 groups, the average egg weight and rate of broken or soft egg were not significantly different ( P>0.05) , and the diet added 0.03% Perilla fru?tescens seed extracts significantly improved laying rate and average daily egg production of laying hens ( P<0.01). 2) The diets added 0.01%, 0.02% and 0.03% Perilla frutescens seed extracts significantly increased the contents of progesterone ( day 15) and estradiol ( days 15 and 30) in serum of laying hens ( P<0.01) , and the diet added 0. 03% Perilla frutescens seed extracts significantly decreased testosterone content in serum of laying hens ( P<0.01) . 3) The diets added 0.01%, 0.02% and 0.03% Perilla frutescens seed extracts signifi?cantly increased interleukin?2 (IL?2) content in serum of laying hens (P<0.01), the diets added 0.02% and 0.03% Perilla frutescens seed extracts significantly increased immunoglobulin G ( IgG) content in serum of laying hens ( P<0.01) , the diet added 0.03% Perilla frutescens seed extracts significantly increased immuno?globulin A ( IgA) content in serum of laying hens ( P<0.01) . These results indicate that diet added 0.03% Pe?rilla frutescens seed extracts can significantly increase the laying rate, average daily egg production and im?mune function of laying hens during the late laying peak period.%为了探讨在饲粮中添加不同水平紫苏籽提取物对蛋鸡产蛋高峰后期生产性能、生殖激素及免疫功能的影响,本试验选用体重、产蛋率基本一致的48周龄海兰褐蛋鸡480只,采用单因素完全随机试验设计,随机分为4组,每组6个重复,每个重复20只. 对照组饲喂基础饲粮,试验Ⅰ、Ⅱ、Ⅲ组分别在基础饲粮中添加0. 01%、0. 02%、0. 03%的紫苏籽提取物,试验期30 d.结果表明:1)与对照组相比,饲粮中添加0.01%、0.02%、0.03%紫苏籽提取物均能降低料蛋比,但差异不显著( P>0.05) ,4组间日均采食量、破软蛋率无显著差异( P>0.05) ,添加0.03%紫苏籽提取物能极显著提高蛋鸡产蛋率和日均产蛋量( P<0. 01 );2 )与对照组相比,添加 0. 01%、0.02%、0.03%紫苏籽提取物能极显著提高蛋鸡血清中孕酮(第15天)和雌二醇(第15和30天)的含量( P<0.01) ,添加0.03%紫苏籽提取物能极显著降低蛋鸡血清中睾酮含量( P<0.01);3)与对照组相比,添加0.01%、0.02%、0.03%紫苏籽提取物能极显著提高蛋鸡血清中白细胞介素-2 (IL?2)含量(P<0.01),添加0.02%、0.03%紫苏籽提取物能极显著提高蛋鸡血清中免疫球蛋白G(IgG)含量(P<0.01),添加 0.03%紫苏籽提取物能极显著提高蛋鸡血清中免疫球蛋白 A (IgA)含量(P<0.01). 由此可知,饲粮中紫苏籽提取物添加水平为0.03%时,能极显著提高蛋鸡产蛋高峰后期产蛋率、日均产蛋量和免疫功能.
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