脱氧雪腐镰刀菌烯醇和玉米赤霉烯酮对癌细胞的促增殖和促迁移作用

摘要

本试验旨在研究脱氧雪腐镰刀菌烯醇(DON)和玉米赤霉烯酮(ZEN)对人结直肠腺癌细胞(HCT116细胞)、人肺癌细胞(A549细胞)、人肝癌细胞(HepG2细胞)的促增殖和促迁移作用.设空白对照组,并以赭曲霉毒素A(OTA)作为阳性对照,采用噻唑蓝(MTT)比色法和细胞划痕愈合试验分别检测细胞活力和细胞迁移能力.结果表明:与空白对照组相比,0.080、0.400 μmol/L OTA能显著或极显著增强 HCT116、A549和 HepG2细胞活力(P<0.05或 P<0.01),50.000 μmol/L OTA 则能极显著降低 HCT116、A549和 HepG2细胞活力(P<0.01);DON在低浓度(0.016、0.080 μmol/L)时对HCT116和A549细胞活力无显著影响(P>0.05),但能极显著增强HepG2细胞活力(P<0.01),而在DON中高浓度(2.000、10.000、50.000 μmol/L)时则能极显著降低 HCT116、A549和 HepG2细胞活力(P<0.01);ZEN 浓度在0.016~50.000 μmol/L时能显著或极显著增强 HepG2细胞活力(P<0.05或 P<0.01),ZEN 在高浓度(50.000 μmol/L)时能极显著降低HCT116和A549细胞活力(P<0.01).细胞划痕愈合试验结果表明,与空白对照组相比,1、10 nmol/L OTA、DON和ZEN作用24、48 h能极显著促进HepG2细胞迁移(P<0.01).由此可见,在0.016~50.000 μmol/L浓度内,DON和ZEN对HepG2细胞有显著的促进增殖和迁移的作用,表明DON和ZEN在HepG2细胞系上具有潜在的致癌性.%This experiment was conducted to study the effects of deoxynivalenol(DON)and zearalenone (ZEN)on promoting proliferation and promoting migration of human colon carcinoma cell(HCT116 cell), human lung cancer cell(A549 cell)and human hepatocellular carcinoma cell(HepG2 cell). The experiment set a control group, and ochratoxin A(OTA)was set up as a positive control, methyl thiazolyl tetrazolium (MTT)colorimetry and cell wound healing experiment were used to determine cell viability and cell migration activity. The results showed that compared with the control group,0.080 and 0.400 μmol/L OTA significantly enhance the viability of HCT116, A549 and HepG2 cells(P<0.05 or P<0.01),50.000 μmol/L OTA could significantly decrease the viability of HCT116, A549 and HepG2 cells(P<0.01). DON had no effect on the viability of HCT116 and A549 cells at low concentration(0.016 and 0.080 μmol/L)(P>0.05),but it could significantly enhance the viability of HepG2 cell at low concentration(0.016 and 0.080 μmol/L)(P<0.01), and significantly decrease the viability of HCT116, A549 and HepG2 cells at middle and high concentration (2.000,10.000 and 50.000 μmol/L)(P<0.01). but significantly enhance the viability of HepG2 cell at the concentration of 0.016 to 50.000 μmol/L(P<0.05 or P<0.01),ZEN could significantly decrease the viability of HCT116 and A549 cells at high concentration(50.000 μmol/L)(P<0.01). The cell wound healing exper-iment results showed that compared with the control group,1 and 10 nmol/L OTA,DON and ZEN could sig-nificantly promote the migration of HepG2 cell at 24 and 48 h(P<0.01). In conclusion, at the concentration of 0.016 to 50.000 μmol/L, DON and ZEN significantly promote proliferation and migration of HepG2 cell, which suggests that DON and ZEN exhibit carcinogenesis-like properties in HepG2 cell.