砷对大鼠附睾中肝细胞生长因子及其受体c-met表达与透明质酸酶活性的影响

摘要

目的:研究砷对大鼠附睾中肝细胞生长因子(HGF)及其受体c-met与透明质酸酶(HYD)活性的影响.方法:SD大鼠随机分为4组,对照组饮用蒸馏水,低、中、高剂量组分别饮用含2.4、12.0、60.0 mg/L亚砷酸钠的蒸馏水.采用免疫印迹法检测HGF、c-met蛋白在大鼠附睾中的表达,改良HYD一明胶底物膜法测精子顶体内HYD活性(HYD阳性反应率、HYD活性强度),扫描电镜观察大鼠精子超微结构的改变.结果:与对照组比较,砷中毒各组HGF、c-met蛋白表达水平均下降,差异有统计学意义;砷染毒中、高剂量组的HYD活性与对照组比较均显著下降.扫描电镜结果显示,与对照组比较,砷染毒低、中、高剂量组大鼠精子顶体完整率均较低,而精子畸形率均较高,差异均有统计学意义.结论:慢性砷中毒能够影响HGF及其受体c-met的表达,使HYD活性减弱,精子顶体完整率下降,精子畸形率升高.%Objective:To study expressions of hepatocyte growth factor (HGF) and c-met in the epididymis of aresnism rats and to investigate the activity of hyaluronidase in sperm of aresnism rats.Methods:Sprague-Dawley rats were randomly divided into four groups,the high dose arsenic infected group (60.0 mg/L),the middle dose arsenic infected group (12.0 mg/L),the low dose arsenic infected group (2.4 mg/L),and the control group (distilled water group).Protein expression levels of HGF and c-met were observed by Western blotting.The postive reaction rate and activity intensity of hyaluronidase (HYD) were detected by modified sodium hyaluronate-gelation membrane.The ultrastrure changes in sperm of rats was observed by scanning electron microscopy (SEM).Results:Compared with control group,the protein expression levels of HGF and c-met in each infected group were significantly lower.Compared with the control group,the positive reaction rate and activity of HYD were significantly lower in the middle and high dose arsenic exposure groups.SEM results of sperm showed that compared with the control group,the sperm acrosome rates in the low,middle and high dose arsenic exposure groups were lower,the sperm malformation rates were higher,and the differences were statistically significant.Conclusion:Arsenism affects the expression of HGF and c-met in the epididymis.The HYD activity is reduced,and with the increase of arsenic concentration,the rates of sperm acrosome intact are decreased,while the rates of sperm deformity are increased.