A reliable cell quenching and metabolite extraction method was crucial for microhial metabolism analysis. For metabolism analysis of Vibrio parahaemolyticus, liquid nitrogen and 75 % methanol (-80 ℃ ) were compared for their efficiency of cell quenching, and six different extraction solvents based on (I) chloroform, (ii) 75% cold methanol. (iii) water, (iv) methanol: chloroform; watcr=10:3:1 (V/V) , (v) methanol: chloroform: water = 3 = 1 = 1 (V/V) and (vi) methanol : chloroform = 1:1 (V/V) were compared for their efficiency of metabolites extracting., The metabolites were detected by GC-MS. The results showed that leakage of metabolite occurred when quenched with 75% methanol ( - 80 t); however this phenomenon did not occur with liquid nitrogen. 47 metabolites were found when extracted with 75% cold methanol, and the relative standard deviation of peak area was 8. 0%. In contrast, 40 or less metabolites were detected with other extraction methods, and the reproducibilities of those methods were unsatisfactory. For global analysis, quenching with liquid nitrogen and extraction with 75% cold methanol was very suitable for metabolomics studies of Vibrio parahaemolyticus.%以气相色谱-质谱联用(GC-MS)为分析方法,比较了液氮和75％甲醇(-80℃)两种溶液对副溶血性弧菌细胞的猝灭效果,以及氯仿、75％冰甲醇、水、甲醇-氯仿-水(10∶3∶1,V/V)、甲醇-氯仿-水(3∶1∶1,V/V)、甲醇-氯仿(1∶1,V/V)6种提取剂对副溶血性弧菌代谢物组的提取效果.结果表明,用75％甲醇(-80℃)猝灭副溶血性弧菌时,出现了代谢物泄漏现象,而液氮猝灭则不存在这个现象；检索发现,采用75％冰甲醇提取获得了47种代谢物,峰面积标准偏差为8.02％,其它5种提取剂获得代谢物种类少于40种,且重现性差.比较色谱峰数量、面积和重现性后发现,液氮猝灭、75％冰甲醇提取适于副溶血性弧菌代谢物组提取.
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