目的:观察ALA-PDT(5-氨基酮戊酸光动力疗法)对兔耳增生性瘢痕形成的抑制作用及对转化生长因子β1,(TGF-β1)表达的影响.方法:在兔耳腹侧面做直径1cm大小全层皮肤缺损创面3个/侧,共60个.随机分为以下三组:高浓度ALA-PDT组(n=20):20%ALA介导PDT治疗;低浓度ALA-PDT组(n=20):10%ALA介导PDT治疗;对照组(n=20):不做治疗,待创面自然愈合.在创面形成术后的第6天、第13天分别进行ALA-PDT治疗,观察治疗组与对照组之间的瘢痕形成率、瘢痕增生指数、胶原纤维含量及TGF-β1表达情况的差异.结果:ALA-PDT治疗降低了瘢痕形成率,其中药物高浓度ALA-PDT组与对照组的差异有统计学意义(P＜0.01);药物高浓度ALA-PDT组和药物低浓度ALA-PDT组的瘢痕增生指教、胶原纤维含量与对照组的差异有统计学意义(P＜0.05);药物高浓度ALA-PDT组与对照组之间的TGF-β1的表达差异具有统计学显著性(P＜0.05).结论:ALA-PDT对兔耳增生性瘢痕的形成有抑制作用,从而预防创伤后增生性瘢痕形成,该作用可能通过减少TGF-β1的产生而实现.%Objective To investigate the ability of ALA-PDT (5-Aminolaevulinic Acid-based Photodynamic Therapy)in reducing hypertrophic scar formation in a rabbit ear model and further to observe the expression of TGF-β1 (transforming growth factor-β1). Methods The acute dermal wound models on white rabbits' ears were established. 60 scars were randomly divided into 3 groups: the high concentration ALA experimental group (n=20) received 20％ALA PDT treatment while the low one (n=20) received 10％ALA PDT treatment, and the controlled group (n=20) received no special treatment. The experimental groups received ALA-PDT treatment at two time points postwounding:days 6,13.Specimens were harvested from scars on postoperative 28 days and scar elevation index, fiber collagen contents were measured,TGF -β1 expression was tested by immunochemical staining. Results Compared with the controlled group,scar formation rate in high concentration ALA-PDT treatment group was reduced significantly (P＜0.05) and the hypertrophic index, fiber collagen contents and the expression of TGF-β1 in both treatment groups were also decreased (with P＜0.05 in both groups),there was no significant difference between high and Iow concentration ALA-PDT treatment groups. Conclusion ALA-PDT may play a role in inhibiting hyperplastic scar formation in rabbit ear,wich probably acted by reducing the expression of TGF-β1.
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