家族聚集性高血压Wnt信号通路基因表达的研究

摘要

Objective To determine the role of gene expression of Wnt signal pathway in the pathogenesis of familial aggregated hypertension.Methods The patients having directly related family members for more than three generations suffering from hypertension were enlisted in the hypertension group,and healthy individuals served as control group.The real-time polymerase chain reaction(PCR)gene array was used to detect the expression of functional classification genes of Wnt signal pathway in peripheral blood,with standard value deviated>2.0 from hypertension group/control group as differential genes.Results When hypertension group was compared with the control group,there were 6 differentially expressed genes,with 5 genes up-regulated,including Bcl-9,microphthalmia associated transcription factor(Mitf),secreted frizzled-related protein-1 (Sfrp-1),Wnt inhibiting factor-1(Wif-1)and ribosomal protein-113a(Rp-113a).There was 1 gene down-regulated,i.e.diekkopf homolog-3(Dkk-3).Conchsion The result of this study suggested that the Wnt signal pathway may be related to the occurrence and development of the familial aggregated hypertension.%目的 探讨Wnt信号通路是否参与家族聚集性高血压的形成.方法 选择3代以上直系亲属具有原发性高血压病史的患者作为观察组,并选择健康体检者作为对照组.采用实时定量聚合酶链反应(PCR)芯片检测外周血Wnt信号通路功能分类基因的表达.采用△△Ct方法,以观察组/对照组基因表达大于2.0倍的基因为差异表达基因.结果 与对照组相比,观察组Wnt信号通路功能分类基因表达大于2.0倍的基因共有6条,其中5条上调,1条下调.上调基因包括Bcl-9、小眼畸形相关转录因子(Mitf)、分泌性卷曲相关蛋白-1(Sfrp-1)、Wnt抑制因子-1(Wif-1)、核糖体蛋白-113a(Rp-113a);下调基因包括Dickkopf同系物-3(Dkk-3).结论 Wnt信号通路参与了家族聚集性高血压的发生发展.