为研究生长因子在过度使用导致肌腱退行性变中的作用机制，利用SD大鼠8周下坡跑模型造成肱二头肌长头肌腱结节沟段退行性变，采用免疫组织化学等方法，检测运动各阶段肌腱 IGF1、TGFβ1、BMP12、bFGF蛋白表达情况。结果：在肱二头肌长头整体肌腱中，只有bFGF表达显著增加；bFGF在过度使用第2周显著升高（ P＜0．01），第4周下降，第6周（ P＜0．05）和第8周（ P＜0．01）再次持续升高。在肱二头肌长头肌腱结节沟段，IGF1表达无显著变化，TGFβ1、bFGF、BMP12表达均显著增高；TGFβ1表达逐渐升高，第6周达到高峰（ P＜0．05），第8周迅速下降；bFGF 表达在第4周迅速升高到峰值（ P＜0．01），然后下降，到第8周与对照组无显著差异；BMP12蛋白表达逐渐增加，第6周达到高峰（P＜0．05），第8周略下降。结论：肌腱过度使用中相关生长因子蛋白长期异常表达是肌腱退行性变的重要病理变化，有可能推动了肌腱退行性变的进程。%Objective :This experiment was designed to study the role of growth factors in tendon degeneration overuse-induced .Methods :This study established the rats tendon overused model by downhill running ,detected growth factors gene expression in various stages of overuse by immunohistochemistry .Results :In whole tendon ,bFGF protein expression was significantly in-creased during the downhill running ,but TGFβ1 ,bFGF and BMP12 had no significantly change .bFGF was significantly increased at 2 weeks ( P< 0 .01 ) ,decreased at 4 weeks ,and raised again at 6-8 weeks .In rat biceps brachii tendon intertubercular sulcus parts ,IGF1 ex-pression had no significantly change ,but expression of TGF β1 ,bFGF ,and BMP12 were sig-nificantly increased .The expression of TGF β1 increased gradually ,reached a peak at 6 weeks (P< 0 .05) ,decreased rapidly at 8weeks .The expression of bFGF reached the peak at 4 weeks value (P<0 .01) ,and then decreased at 8 weeks .The expression of BMP12 protein in-creased gradually ,reached a peak at 6 weeks ( P< 0 .05 ) ,and declined slightly at 8 weeks . Conclusion :During overuse process ,long-term abnormal expression of growth factors was an important pathological chang of tendon degeneration ,which probably pushed the tendon degen-eration process .
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