野葛、粉葛与云南葛中葛根素、大豆苷、大豆苷元、染料木素的含量比较

摘要

目的:比较野葛、粉葛与云南葛中葛根素、大豆苷、大豆苷元和染料木素的含量.方法:样品以30％乙醇回流提取后采用高效液相色谱法测定含量.色谱柱为Uvis-201 (250 mm×4.6 mm,5 μm),流动相为甲醇-水(梯度洗脱),流速为1.0 mL· min-1,检测波长为250 nm,柱温为室温.结果:葛根素、大豆苷、大豆苷元、染料木素的进样量分别在0.68～5.44 μg(r＝0.999 2)、0.143～1.144 μg(r＝0.999 3)、0.024 5～0.196 0 μg(r＝0.999 2)、0.088～0.704 μg(r＝0.999 4)范围内与各自峰面积积分值呈良好线性关系；平均加样回收率分别为98.1％ 、99.5％、101.1％、99.2％,RSD分别为2.17％、2.42％、2.53％、2.65％(n均为6).葛根素含量高低排序为河南野葛＞云南葛＞山西野葛＞大别山野葛＞广西粉葛；大豆苷含量为云南葛＞河南野葛＞大别山野葛＞山西野葛＞广西粉葛；大豆苷元含量为河南野葛＞云南葛＞山西野葛＞大别山野葛＞广西粉葛；染料木素含量为河南野葛＞云南葛＞山西野葛＞大别山野葛＞广西粉葛.结论:野葛、粉葛与云南葛中葛根素、大豆苷、大豆苷元、染料木素含量差别较大,且云南葛为野葛的伪品之一,三者不能同等应用.%OBJECTIVE: To compare the content of puerarin, daidzin, daidzein and genistein from Pueraria lobata, P. thomsonii and P. peduncularis. METHODS: After reflux extraction of 30% ethanol, the sample was determined by HPLC. The determination was performed on Uvis-201 (250 mm×4.6 mm, 5 μm) column with mobile phase consisted of methanol-water (gradient elu-tion) at the flow rate of 1.0 mL·min-1. The detection wavelength was 250 nm and column temperature was ambient temperature. RESULTS: The linear range was 0.68-5.44 μg for puerarin(r= 0.999 2) ,0.143-1.144 μg for daidzin (r=0.999 3), 0.024 5-0.196 0 μg for daidzein(r=0.999 2) and 0.088—0.704 μg(r=0.999 4) for genistein; average recovery rates were 98.1% ,99.5%, 101.1% and 99.2%,RSDs were 2.17% ,2.42% ,2.53% and 2.65% (n=6). The content of puerarin was in descending order :P. lobata in Henan>P. peduncularis>P. lobata in Shanxi>P. lobata in Dabieshan>P. thomsonii in Guangxi; the content of daidzin was in descending order:P. peduncularis>P. lobata in Henan>P. lobata in Dabieshan>P. lobata in Shanxi>P. thomsonii in Guangxi; the content of daidzein was in descending order:P. lobata in Henan>P. peduncularis>P. lobata in Shanxi>P. lobata in Dabie-shan>P. thomsonii in Guangxi; the content of genistein was in descending order: P. lobata in Henan>P. peduncularis>P. lobata in Shanxi>P. lobata in Dabieshan>P. thomsonii in Guangxi. CONCLUSION: The contents of puerarin, daidzin, daidzein and genistein in P. lobata, P. thomsonii and P. peduncularis were different greatly, and P. peduncularis is a false one of P. lobata. The use of them should be different.