HPLC法测定降糖消脂片中盐酸小檗碱的含量

摘要

目的：建立测定降糖消脂片中盐酸小檗碱含量的方法。方法：采用高效液相色谱法。色谱柱为Symmetry C18，流动相为乙腈-0.05 mol/L磷酸二氢钠溶液（用磷酸调节pH至3.0）（24∶76，V/V），流速为1.0 ml/min，检测波长为345 nm，柱温为室温，进样量为10μl。结果：盐酸小檗碱进样量在0.522～4.698μg范围内与其峰面积呈良好的线性关系（r＝0.9999）；精密度、稳定性、重复性试验的RSD≤0.72％；平均加样回收率为97.79％，RSD为2.09％（n＝6）。结论：该方法简便、准确、灵敏、重复性好，适用于降糖消脂片中盐酸小檗碱的含量测定。%OBJECTIVE:To establish a method for the content determination of berberine hydrochloride in the Jiangtang xiao-zhi tablets. METHODS:HPLC was conducted with the Symmetry C18 column. The mobile phase was acetonitrile-0.05 mol/L sodium dihydrogen phosphate(pH adjusted to 3.0 using phosphoric acid)(24∶76,V/V)at the flow rate of 1.0 ml/min. The detection wave-length was 345 nm,the column temperature was room temperature and injection volume was 10 μl. RESULTS:The linear range of berberine hydrochloride was 0.522-4.698 μg(r=0.999 9);RSDs of precision,stability and reproducibility tests were no more than 0.72%;the average recovery was 97.79%(RSD=2.09%,n=6). CONCLUSIONS:The method is simple,accurate,sensitive and reproducible,and can be used for the content determination of berberine hydrochloride.