HPLC法测定尪痹片中蛇床子素和二氢欧芹醇当归酸酯含量

摘要

目的：建立同时测定尪痹片中蛇床子素和二氢欧芹醇当归酸酯含量的高效液相色谱法。方法：色谱柱：Agilent XDB C18(150 m ×4.6 mm,5μm)；流动相：乙腈-水(49∶51)等度洗脱；流速：1.0 ml·min-1；检测波长：330 nm。结果：蛇床子素在0.060~0.597μg范围内线性关系良好(r=0.9999),平均回收率为99.12%；二氢欧芹醇当归酸酯在0.010~0.102μg范围内线性关系良好(r=0.9999),平均回收率为98.98%。结论：本方法简便、准确,重复性好,可用于尪痹片中上述两种成分的含量测定。%Objective:To establish an HPLC method for the determination of osthole and columbianadin in Wangbi tablets. Meth-ods:The column was an Agilent XDB C18(150 mm ×4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-water(49∶51)with gradient elution. The flow rate was 1. 0 ml·min-1 and the detection wavelength was at 330 nm. Results: The calibration curve was linear within the range of 0. 060-0. 597 μg(r=0. 999 9) for osthole and 0. 010-0. 102 μg(r=0. 999 9) for columbianadin. The aver-age recovery was 99. 12% and 98. 98%, respectively. Conclusion:The method is simple, practicable, accurate and rapid. It can be applied in the content determination of osthole and columbianadin in Wangbi tablets.