目的: 建立HPLC-ELSD同时测定咳灵胶囊中的苦杏仁苷、桃叶珊瑚苷、哈帕苷、贝母辛、贝母素甲和贝母素乙含量的方法. 方法: 采用Ultimate XB C 18 色谱柱(250 mm× 4.6 mm,5 μm),蒸发光散射检测器(漂移管温度105 ℃,氮气流速 2.0 L·min-1);以乙腈-甲醇(1∶1)与 0.4% 醋酸溶液为流动相,进行梯度洗脱,流速: 0.7 ml·min-1,柱温:35 ℃. 结果: 苦杏仁苷、桃叶珊瑚苷、哈帕苷、贝母辛、贝母素甲和贝母素乙的线性范围分别为13.56 ~ 271.20 μg·ml-1 (r=0.999 2)、 8.48 ~ 169.60 μg·ml-1 (r=0.999 9)、 4.89 ~ 97.80 μg·ml-1 (r=0.999 7)、 2.66 ~ 53.20 μg·ml-1 (r=0.999 4)、 1.82 ~ 36.40 μg·ml-1 (r=0.999 8)、 2.04 ~ 40.80 μg·ml-1 (r=0.999 6);平均加样回收率(RSD)分别为97.90% (1.20%), 99.21% (1.62%), 97.68% (0.75%), 98.36% (1.38%), 99.70% (0.79%), 97.95% (1.56%)(n=6).结论: 本文建立的方法结果准确,重复性好,样品处理简便,可作为咳灵胶囊的质量控制方法.%Objective: To develop an HPLC-ELSD method for the determination of amygdalin, aucubin, harpagide, peimisine, peimine and peiminine in Keling capsules simultaneously.Methods: An Ultimate XB C 18 (250 mm× 4.6 mm , 5 μm) chromatographic column was adopted with an ELSD (the drift tube temperature was 105℃, the flow rate of nitrogen was 2.0 L·min-1).The mobile phase was acetonitrile-methanol (1∶1) and 0.4% acetic acid solution with gradient elution at a flow rate of 0.7 ml·min-1 , and the column temperature was set at 35 ℃.Results: Amygdalin, aucubin, harpagide, peimisine, peimine and peiminine was linear within the range of 13.56-271.20 μg·ml-1 (r=0.999 2), 8.48-169.60 μg·ml-1 (r=0.999 9), 4.89-97.80 μg·ml-1 (r=0.999 7), 2.66-53.20 μg·ml-1 (r=0.999 4), 1.82-36.40 μg·ml-1 (r=0.999 8) and 2.04-40.80 μg·ml-1 (r=0.999 6), respectively.The average recovery and the corresponding RSD were 97.90% (1.20%), 99.21% (1.62%), 97.68% (0.75%), 98.36% (1.38%), 99.70% (0.79%) and 97.95% (1.56%)(n =6), respectively.Conclusion: The method is simple and specific, and the results are accurate and repeatable.The method is helpful to the quality control of Keling capsules.
展开▼
