雷公藤不同乙醇提取物指纹图谱及与大鼠肾小球系膜细胞的谱效关系研究

摘要

目的:探讨雷公藤不同乙醇提取物中对大鼠肾小球系膜细胞(GMC)有增殖影响的活性成分.方法:采用HPLC法建立5个不同乙醇浓度雷公藤提取物的指纹图谱,选择大鼠肾小球系膜细胞模型进行药效学研究,考察不同乙醇浓度雷公藤提取物对大鼠肾小球系膜细胞的增殖影响.采用偏最小二乘回归分析法分析谱效关系.结果:建立了5个不同乙醇浓度雷公藤提取物的HPLC指纹图谱,并从HPLC指纹图谱中提取出32个能够表征含量差异的特征峰.药效研究结果表明,60 %、70 %、95 %乙醇提取物和雷公藤多苷片组均呈现一定的量效关系,随着给药量增加,细胞增殖的抑制作用越大.中、高剂量的60%提取物组的吸光度值均低于同剂量其他各提取物.采用偏最小二乘回归分析法以大鼠肾小球系膜细胞增殖抑制率为"药效"指标时,高剂量(40 μg·L-1)下有20个峰为药效峰;中剂量(20 μg·L-1)下有17个峰为药效峰;低剂量(10 μg·L-1)下有15个峰为药效峰.结论:部分药效峰随着给药剂量的变化,呈现一定规律的量效关系.%Objective:To investigate the proliferation of rat glomerular mesangial cells (GMC) influenced by different ethanol extracts of Tripterygium wilfordii HooK F.(TWHF).Methods:An HPLC method was used to establish the fingerprints of 5 different ethanol extracts of TWHF,and GMC was chosen to study the effects of different ethanol extracts of TWHF on cell proliferation.After statistical analysis,the spectrum-activity relationship was analyzed by using partial least squares regression(PLSR).Results:The HPLC fingerprints of the 5 different ethanol extracts of TWHF were established,and 32 characteristic peaks were characterized by the HPLC fingerprints.60%,70% and 95% ethanol extracts and glycosides tablets showed dose-effect relationship,and with the increase of dose,the more significant inhibition of cell proliferation was exhibited.The absorbance values of the 60% ethanol extracts at medium and high doses were lower than those of the other extracts at the same dose.The proliferation inhibition rate of GMC was used as the potency index and analyzed by PLSR,and 20 peaks were potency peaks at high dose(40 μg·L-1),17 ones were potency peaks at medium dose(20 μg·L-1) and 15 ones were potency peaks at low dose(10 μg·L-1).Conclusion:Part of the potency peaks has regular dose-effect relationship with the changes of dose.