Objective A method for determination of aflatoxin B1, B2,G1, G2 using immunoaffinity column clean - up and HPLC with post - column photochemical derivatization was developed. Methods The samples were extracted with methanol - water solution (80 ∶ 20) , and cleaned up by immunoaffinity columns. The separation of aflatoxin B1,B2,G1,G2 were conducted by HPLC and the determination was carried out by fluorescence detector after photochemical derivatization. Results Under optimum conditions,the limits of detection of aflatoxin G2,G1, B2, B1 were 0. 15 ,0. 25 ,0. 1 , 0. 2 μg/kg respectively , and the recoveries of analytes were 78. 8％ - 106. 7％ , RSD was below 7. 1 ％ . Conclusion The method is simple,high sensitive and good repeatability, which is suitable for the determination of aflatoxins in animal medicines.%目的 建立动物类药材中黄曲霉毒素B1,B1,G1,C2的免疫亲和柱净化光化学衍生高效液相色谱-荧光检测(HPLC-FLD)法.方法 样品经甲醇-水(80∶20)提取后,通过免疫亲和柱净化、柱后光化学衍生、高效液相色谱-荧光检测器测定.结果 在优化条件下,黄曲霉毒素G2,G1,B2,B1的检出限分别为0.15,0.25,0.1,0.2 μg/kg,回收率为78.8%～106.7%,RSD均低于7.1%.结论 所用方法简便快速、灵敏度高、重现性好,可满足动物类药材中黄曲霉毒素检测的需要.
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