注射用青霉素钠与脂肪乳的配伍稳定性及体外抑菌活性检测

摘要

Objectives To investigate the stability and in vitro antimicrobial activity in compatibility of benzylpenicillin sodium injection with 10% ,20% and 30% lipid emulsion, respectively. Methods The pH meter and the Zetasizer Nano ZS particle size analyzer were adopted to detect the pH value and the particle size of the compatible solution within 24 h at 25 ℃ and 4 ℃. The benzylpenicillin sodium in the compatible solution were determined by high performance liquid chromatographyC HPLC A The in vitro antimicrobial activity of compatible solution was investigated by the microdilution method. Results After compatibility of benzylpenicillin sodium injection with 10% ,20% and 30% lipid emulsion, there were no significant changes in the appearance, pH value, particle size and content of the compatible solution. The antimicrobial action of benzylpenicillin sodium was not affected. Conclusion The compatible solution of benzylpenicillin sodium injection respectively mixed with 10% ,20% and 30% lipid emulsion has good stability and conforms to the requirement of injection. So this compatibility may be applied.%目的 考察注射用青霉素钠分别与10%,20%,30%脂肪乳配伍后的稳定性及体外抑菌活性.方法 分别利用pH计和动态光散射粒径仪检测配伍液在25℃和4℃下24 h内pH和粒径的变化情况,采用高效液相色谱法检测配伍液中药物的含量变化情况,采用微量液体稀释法考察配伍液的体外抑菌活性.结果 注射用青霉素钠分别与10%,20%,30%脂肪乳配伍后,配伍液的外观、pH、粒径、含量均无明显改变,配伍后对青霉素钠的抑菌作用也无影响.结论 注射用青霉素钠与10%,20%,30%脂肪乳配伍后,稳定性良好,符合注射液的要求,可以配伍使用.