目的 建立奥美沙坦血药浓度的高效液相色谱法.方法 以盐酸哌唑嗪为内标,血浆样品用沉淀法,色谱柱为奥泰Apollo C18柱(150 mm×4.6mm,5μm),在线过滤器；以0.1％三乙胺水溶液(甲酸调pH为2.5)-乙腈(75:25)为流动相；激发波长为260 nm,发射波长为379 nm;流速为1.0 mL/min,柱温为25℃.结果 奥美沙坦质量浓度线性范围是10.00 ～1 280.00 ng/mL(r =0.999 6),最低检测浓度为10.00 ng/mL,方法的相对回收率为97.53％ ～99.24％ (n=5),绝对回收率为86.89％ ～ 89.32％(n=5),日内RSD为1.25％ ～ 2.26％,日间RSD为1.14％～5.55％.结论 该方法简便、准确、灵敏,特异性强,重复性好,可用于血浆中奥美沙坦的测定及药代动力学研究.%Objective To establish a HPLC method for the determination of olmesartan in human plasma.Methods Prazosin hydrochloride was used as the internal standard.The plasma sample adopted by the precipittion method.The Apollo C18 column (150mm×4.6 mm,5 μm) was used as the analytical column with the mobile phase of 0.1％ triethylamine (adjusting pH to 2.5 with formic acid)-acetomitrile (75:25).The excitation wavelength(λex) of 260 nm and emission wavelength of (λem) 379 nm.The flow rate was 1.0 mL/min.The column temperature was 25 ℃.Results A good linear relation was obtained within the plasma olmesartan concentration range of 10.00-1 280.00 ng/mL(r =0.999 6).The lowest detection concentration was 10.00 ng/mL,the relative recovery rate was 97.53％-99.24％ (n =5) and the absolute recovery rate was 86.89％-89.32％ (n =5); the within-day RSD and the between-day RSD were 1.25％-2.26％ and 1.14％-5.55％ respectively.Conclusion This method is shown to be simple,accurate and sensitive with strong specificity and good reproducibility,and can be used for the determination of olmesartan level in human plasma and the pharmacokinetic study of olmesartan.
展开▼
