复方灵芝孢子精油软胶囊抗疲劳增强免疫力作用研究

摘要

目的：研究复方灵芝孢子精油软胶囊抗疲劳、增强免疫力的药理作用。方法将昆明种小鼠随机分为5组，分别为复方灵芝孢子精油软胶囊（FFLZB）低、中、高[385，770，1540 mg／(kg·d)]剂量组、对照药红参醇提物组、空白对照组，灌胃给药14 d后考察各组小鼠爬杆时间及爬杆后血清肌酸激酶（CK）、三磷酸腺苷（ATP）水平，同时采用免疫脏器指数法、碳粒廓清法、足趾增厚法测定迟发型变态反应（DTH），考察FFLZB对小鼠免疫功能的影响。结果高、中、低剂量FFLZB能不同程度地延长小鼠爬杆时间，高、中剂量能显著提高小鼠爬杆后血清ATP水平，仅低剂量能使爬杆后的小鼠血清CK酶水平显著提高；与空白对照组相比，FFLZB高、中、低3个剂量能不同程度地抑制小鼠免疫器官脏器指数的下降，提高碳粒廓清指数水平及24，48 b迟发型变态反应程度。结论复方灵芝孢子精油软胶囊具有抗疲劳及增强免疫力作用，是一种有效的抗疲劳复方中药制剂。%Objective To researcb tbe pbarmacological effect of antifatigue and immunopotentiation of Compound Ganoderma Lucidum Spore Oil Soft Capsule. Methods Kunming mice were radomly devided into five groups:tbe low,middle and bigb FFLZB doses groups [ 385,770,1 540 mg/ ( kg·d)] ,control red gingseng etbanol extract group and blank control group. Tbe pole-climbing time,serum cre-atinine kinase(CK)and adenosine tripbospbate(ATP)contents on 14 d after medication gavage were detected. At tbe same time tbe immune organ index metbod,cleaning carbon particle metbod and digits tbickening metbod were adopted to detect delayed type bep-ersensitivity(DTH)for investigating tbe influence of CGOC on mice′s immune function. Results Tbe bigb,middle and low doses FFLZB groups could prolong tbe pole-climbing time in different degrees,tbe middle and bigb doses groups could significantly increase tbe serum ATP level after pole-climbing,only tbe low dose group could significantly increase serum CK level after pole-climbing;com-pared witb tbe blank control group,tbe bigb,middle and low FFLZB doses groups could inbibit tbe decrease of mice immune organ in-dex in different degrees,increased tbe level of leaning carbon particles and 24,48 b DTH degree. Conclusion FFLZB bas tbe an-tifatigue and immunopotentiation effect and is a compound Cbinese medicine preparation for effective antifatigue.