Exploring the Degradation Potential of Halomonas Bacteria from Oil-contaminated Marine Environment

摘要

The degradation potential of a diesel-degrading bacteria (HDMP1) isolated from the oil-contaminated marine environment was explored systematically by analyzing the environmental conditions and synergistic action with other die-sel-degrading bacteria. The result indicated that HDMP1 was confirmed as a strain of Halomonas by the method of strain identification. And, HDMP1 showed good diesel degradation performance with a diesel degradation rate of up to 79.59％ after 7 days. By analyzing the effect of environmental conditions on HDMP1, the best carbon and nitrogen sources were found to be lactose and peptone, respectively, at a pH value of 7.5 and a salinity of 4 g/(100 mL). Additionally, the synergis-tic effect of HDMP1 combined with other diesel-degrading bacteria was analyzed by orthogonal experimental design. The inocula of HDMP1, HDMP2, HDMP3 and HDMB3 were optimized, with the best results equating to 0.4％, 0.1％, 0.4％ and 0.9％ in 100 mL of MSM, respectively, while the degradation rate of diesel was identified to be 73.5％ within 5 days in the presence of optimum inocula.