血清总免疫球蛋白E和特异性免疫球蛋白E联合检测在小儿喘息性疾病诊断中的应用价值

摘要

Objective To study the application value of detection of total serum immunoglobulin E (tIgE) and specific immunoglobulin E(sIgE) in diagnosis of children with respiratory allergic disease. Methods 60 Children with respiratory allergic disease admitted into our hospital from September 2011 to September 2014 were selected as observation group, another 30 cases of healthy children was as control group.The observation group had already been diagnosised as respi-ratory allergic disease.Serum tIgE and sIgE was measured by enzyme linked immunosorbent assays,and the relevant re-sults was compared between two groups. Results Levels of serum tIgE and sIgE in the observation group respectively was (501±219) U/ml and (3450±1240) U/L,which was remarkably higher than that of control group,the difference was significant(P<0.05).In the 60 children patients,merely 65%(36/60) showered higher levels of serum tIgE,but 45%(27/60) was found to be clinically symptomatic.The sensitivity of used tIgE was 67%,the sIgE was 65%,combination was 78%, the area of ROC curve below was 89%. Conclusion Close correlations exist between immunoglobulin E levels and asthmatic disease in children.Combination detection of tIgE and sIgE may increase the detection rate of asthmatic dis-ease in children,it is worthy of clinic application.%目的：研究血清总免疫球蛋白E(tIgE)和特异性免疫球蛋白E(sIgE)联合检测在小儿喘息性疾病诊断中的应用价值。方法选择2011年9月~2014年9月本院收治的60例喘息性疾病患儿作为观察组，选取同期体检健康的30例小儿作为对照组，观察组患儿均已经过临床实验检测诊断证实为喘息性疾病。采用酶联免疫法检测两组儿童的血清tIgE、sIgE水平，并将相应结果进行比较。结果观察组患儿的tIgE、sIgE水平分别为(501±219)U/ml、(3450±1240)U/L，显著高于对照组相应水平，两组差异有统计学意义(P<0.05)；60例患儿中，65%(36/60)患儿的血清免疫球蛋白偏高，45%(27/60)有变态反应症状；单独采用tIgE检测的灵敏度为67%，单独采用sIgE检测的灵敏度为65%，两者联合检测的灵敏度为78%，ROC曲线下面积为89.25。结论小儿喘息性疾病与免疫球蛋白水平具有密切的关系，采用血清tIgE和sIgE联合检测可以显著提高小儿喘息性疾病的检出率，值得临床推广应用。