目的:建立 HPLC 法同时测定川芎药材中阿魏酸和咖啡酸含量的方法。方法采用 Kromasil -ODS (250mm×4.6mm,5μm)色谱柱,流动相为甲醇-0.5%醋酸水(35:65);流速:1.0mL/min;检测波长:323nm。结果阿魏酸和咖啡酸的线性范围分别为:13.62~257.4mg/mL(r=0.9996)和29.40~515.7mg/mL (r=0.9995);平均回收率(n=6)分别为阿魏酸96.6%(RSD=0.8%)和咖啡酸95.6%(RSD=0.5%)。结论该法操作简单,结果准确,重现性好,为全面评价不同产地川芎药材的质量提供方法。%Objective To develop an HPLC method for Ferulic acid, Caffeic acid in Rhizoma Chuanxiong. Methods Kromasil -ODS (250mm×4.6mm, 5μm) column was used to separate the sample at the column temperature of 35 ℃ . Methanol was used with a mobile phase of methanol -0.5% aetic acid (35:65, v/v) at a flow rate of 1.0mL/min. The detection wavelengths was set at 323nm. Results The calibration curve was linear over the concentration of 13.62-257.4μg/mL for ferulic acid (r=0.9995). 29.40-515.7μg/mL for Caffeic acid (r=0.9996). The average recovery of Ferulic acid and Caffeic acid were 96.6%, 95.6%, and the RSD were 0.8%, 0.5%, respectively. Conclusion This method is smiple and reproducible, which can be used for the quality control of Rhizoma Chuanxiong.
展开▼