小干扰垂体特异性转录因子-1对大鼠垂体生长激素肿瘤细胞侵袭行为的影响

摘要

Objective: To study the effect of the biological characteristics with the pituitary-specific transcription factor-1 siRNA-transfected rat GH3 Pituitary tumor cells. Methods: Designed synthetic siRNA, liposome (lipofectin) transfected rat pituitary tumor cells by RT-PCR, assayed inhibition of tumor cell pit - 1 mRNA and pit - 1 protein expression results, preparation of Boyden chamber determination of pit-lsiRNA on tumor cell invasion and chemotaxis effects. Results: Pit-1 siRNA-transfected rat GH3 cells after 24 h, pit -1 mRNA expression was significantly lower than the negative control group (P<0.05). Through the porous membrane matrix gel in rat GH3 cells the number of tumor cells were significantly lower than the negative control group (P<0.05). Conclusion: Pit-1 in rat Pituitary adenoma cells (GH3) is positively correlated with aggressive, play an important role with the growth hormone in GH3 Pituitary adenoma cells in the pathogenesis.%目的:研究垂体特异性转录因子-1(pituitary-specific transcription factor-1,pit-1)siRNA 转染大鼠GH3 型垂体瘤细胞后对其生物学特性的影响.方法:设计合成pit-1 siRNA,经脂质体(lipofectin)转染大鼠垂体瘤细胞后,通过RT-PCR 检测瘤细胞内pit-1 mRNA 表达的变化;制备Boyden 小室测定pit-1 siRNA 对肿瘤细胞侵袭力和趋化作用的影响.结果:pit-1 siRNA 转染大鼠GH3 细胞24 h 后,pit-1 mRNA 和pit-1 蛋白的表达明显低于阴性对照组(P<0.05).透过基质凝胶多孔滤膜的大鼠GH3 细胞瘤细胞的数量都显著低于阴性对照组(P<0.05).结论:pit-1 对大鼠垂体瘤细胞的侵袭性呈正相关,在生长激素腺瘤的发病机制中起重要作用.