MMP-2、 MMP-9及VEGF在结肠癌中的表达及其与肿瘤转移的相关性

摘要

目的 探讨基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)及血管内皮生长因子(VEGF)在结肠癌中的表达,并分析其与肿瘤转移的相关性.方法 86例经病理证实的结肠癌患者,采用免疫组化SP法检测MMP-2、MMP-9及VEGF在结肠癌组织及正常结肠组织中的表达,分析三者间的相关性及其与肿瘤转移的相关性.结果 结肠癌组织中MMP-2、MMP-9及VEGF表达水平及阳性率均显著高于正常结肠组织(P＜0.05、0.01),其中有淋巴结转移的结肠癌组织中MMP-2、MMP-9及VEGF阳性表达率高于无淋巴结转移者(P＜0.05)；MMP-2、MMP-9与VEGF表达之间呈显著正相关(r=0.72,P＜0.05).结论 结肠癌组织中MMP-2、MMP-9及VEGF高表达与肿瘤转移密切相关,三者可作为反映结肠癌进展的重要指标,为判断患者的预后提供依据.%Objective To explore the expressions of MMP-2, MMP-9 and VEGF in colon carcinoma, and analyze their correlations with tumor metastasis. Methods 86 patients with colon carcinoma confirmed by pathology were enrolled in our study, and the expressions of MMP-2, MMP-9 and VEGF in colon carcinoma and normal colon tissues were determined by using immunohistochemistry SP assay, the correlations among the three indexes and relationships with tumor metastasis were also investigated. Results In colon carcinoma tissues, the expression levels and positive rates of MMP-2, MMP-9 and VEGF were statistically higher than those of normal colon tissues (P < 0.05, 0.01), patients with lymph node metastasis showed significantly higher positive rates of MMP-2, MMP-9 and VEGF than those of patients without lymph node metastasis (P < 0.05). It showed obvious positive correlation among MMP-2, MMP-9 and VEGF (r = 0.72, P < 0.05). Conclusion The high expressions of MMP-2, MMP-9 and VEGF in colon carcinoma are closely related to tumor metastasis, these three indexes can reflect the progress of colon carcinoma and provide basis for judging the prognosis of patients with colon carcinoma.