当归藤水提物和醇提物急性毒性实验研究

摘要

目的 对当归藤水提取物和醇提取物的急性毒性进行初步研究,评价其安全性,为新药开发提供理论依据.方法 采用最大给药量测定法,小鼠灌胃给药测定当归藤水提取物和醇提物的半数致死量(median lethal dose,LD50)或最大耐受量(maximum tolerated dose,MTD),研究其安全性.结果 当归藤水提取物和醇提取物的毒性都很小,未测得灌胃给药小鼠半数致死量LD50,水提取物和醇提取物的MTD > 90 g/kg,最大耐受量倍数相当成人(50 kg)用量的150倍.结论 当归藤无明显毒性,临床常用量安全可行.%Objective To study the acute toxicity of water extract and ethanol extract of Angelica vine, evaluate their safety and provide theoretical basis for the development of new drugs. Methods The maximum administration determination method was used and the drug was administered orally. The median lethal dose (LD50) and maximum tolerated dose (MTD) of water extract and ethanol extract of Angelica vine were measured to study the safety. Results The toxicity of water extract and alcohol extract of Angelica vine was very small, thus the LD50 of mice could not be obtained. MTD of water extract and ethanol extract was larger than 90 g/kg. The maximum tolerated dose multiple equals 150 times of the dose for adults (50 kg). Conclusion Angelica vine does not have apparent toxicity, thus its clinical routine dose is safe and feasible.