首页> 中文期刊>中国医药导报 >戊四氮致癫痫大鼠大脑皮质区突触体素、缝隙连接蛋白43、神经胶质原纤维酸性蛋白表达水平的变化

戊四氮致癫痫大鼠大脑皮质区突触体素、缝隙连接蛋白43、神经胶质原纤维酸性蛋白表达水平的变化

     

摘要

Objective To explore expression level change of SYN, Cx43 and GFAP in rat cerebral cortex after PTZ-in-duced epilepsy. Methods 30 healthy male SD rats were randomly divided into two groups:control group (n=15) and ex-perimental group (n=15). Control group was peritoneal injected with physiological saline, which had similar capacity with PZT. Brain tissues of experimental group were gained after 28 days intraperitoneal injection with PTZ 35 mg/kg every day. Immunohistochemisty was used to detect the expression of SYN, Cx43 and GFAP. Western blot was used to detect the expression of SYN and Cx43. Results Immunohistochemisty:average absorbance values of SYN (0.66±0.06), Cx43 (0.19±0.05), GFAP (0.61±0.08) of rat cerebral cortex in experimental group were higher than those in control group (0.43±0.05, 0.11±0.03, 0.36±0.05), with statistical differences (P< 0.05). Western blot: expression level of SYN (0.26±0.07), Cx43 (0.93±0.07) of rat cerebral cortex in experimental group were higher than those in control group (0.13±0.04, 0.61±0.06), with statistical differences (P<0.05). Conclusion The expression levels of SYN, Cx43 and GFAP are closely related with occurrence and development of epilepsy, which provides the basis for study of etiology and patho-genesis of epilepsy.%目的:探讨戊四氮致癫痫大鼠大脑皮质区突触体素(SYN)、缝隙连接蛋白43(Cx43)以及神经胶质原纤维酸性蛋白(GFAP)表达水平的变化。方法将30只雄性健康SD大鼠随机分为两组:对照组(n=15)和实验组(n=15)。对照组腹腔注射与戊四氮致癫痫剂量等容量的生理盐水,实验组采用戊四氮35 mg/kg连续腹腔注射28 d后取脑。采用免疫组化染色检测大鼠大脑皮质区SYN、Cx43和GFAP表达的变化,采用Western blot测定大鼠大脑皮质区SYN、Cx43含量的变化。结果免疫组化染色:实验组大鼠大脑皮质区SYN(0.66±0.06)、Cx43(0.19±0.05)、GFAP(0.61±0.08)的平均吸光度值均高于对照组(0.43±0.05、0.11±0.03、0.36±0.05),差异有统计学意义(P<0.05)。Western blot:实验组大鼠大脑皮质区SYN(0.26±0.07)、Cx43(0.93±0.07)的表达水平均高于对照组(0.13±0.04、0.61±0.06),差异有统计学意义(P<0.05)。结论SYN、Cx43和GFAP的表达水平与癫痫的发生及发展密切相关,为研究癫痫的病因及发病机制提供依据。

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