目的 采用反相高效液相色谱法,建立同时测定补骨脂提取物中补骨脂素、异补骨脂素和补骨脂酚含量的方法.方法 采用Epic C18(250 mim×4.6 mm,5μm)色谱柱;流动相:甲醇-水,梯度洗脱;流速:1.0 mL/min;柱温:30℃;检测波长250 nm.结果 该法测定补骨脂素、异补骨脂素和补骨脂酚分别在0.0563~0.3379、0.0523~0.3139、0.1280~0.7680 μg范围内线性关系良好,相关系数均为0.9999.平均加样回收率分别为100.66%(n=6)、RSD=1.77%,100.43%(n=6)、RSD=1.45%和99.41%(n=6)、RSD=1.63%.结论 本方法简便、准确、灵敏、重复性好,可作为补骨脂提取物中补骨脂素、异补骨脂素和补骨脂酚的含量控制方法.%Objective To determine psoralen,isopsoralen and bakuchiol from the extract of Psoralea corylifolia by RPHPLC.Methods The contents of paoralen,isopsoralen and bakuchiol were determined on the Epic C18 (250 mm×4.6 mm,5 μm) column,with methanol:water in gradient elution manner.The flow rate was 1.0 mL/min,the column temperature was maintained at 30℃,and the detection wavelength was set up at 250 nm.Results The linear ranges of paoralen,isopsoralen and bakuchiol were 0.0563-0.3379,0.0523-0.3139 and 0.1280-0.7680 μg (r=0.9999).The average recovery of paoralen,isopsoralen and bakuchiol were 100.66%,100.43% and 99.41%,RSD were 1.77%,1.45% and 1.63% (n=6).Conclusion The method is convenient,accurate sensitive and repeatable,and can be used in the content control method of psoralen,isopsoralen and bakuchiol in extract of Psoralea corylifolia.
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