Ki-67、Bcl-2、Bax和Caspase-3在脑胶质瘤组织中的表达及临床意义

摘要

Objective To investigate the expression levels of Ki-67,Bcl-2,Bax and Caspase-3 in patients with brain glioma and its clinical significance.Methods 65 cases with brain glioma in Affiliated Hospital of Logistics College of PAP from October 2014 to October 2016 were selected and divided into low grade glioma group (34 cases) and high grade glioma group (31 cases) according to the classification of tumors of the nervous system.Another 18 cases with normal brain tissue specimens were selected as the control group.The expression levels of Ki-67,Bcl-2,Bax and Caspase-3 were detected and compared.Besides,their correlations with the malignant degree of glioma were analyzed.Results As the pathological grade of glioma increasing,the expression level of Ki-67 increased.The positive rates in normal brain tissue,low and high grade glioma group were 0,20.6％ and 77.4％,respectively,with statistically significant difference (P ＜ 0.05),and had a positive correlation with pathological grade (Spearman correlation coefficient was 0.645,P ＜ 0.05).The expression level of Bcl-2 increased with the increase of glioma grade (6.2％,73.5％,100％),which showed a positive correlation with pathological grade (Spearman correlation coefficient was 0.711,P ＜ 0.05).The expression level of Bax decreased with the increase of glioma grade (100.0％,82.4％,16.1％),which showed a negative correlation with pathological grade (Spearman correlation coefficient was-0.706,P ＜ 0.05).The expression level of Caspase-3 in low grade glioma group was the highest among groups,which decreased with the increase of pathological grade and showed a negative correlation with pathological grade (Spearman correlation coefficient was-0.334,P ＜ 0.05).Conclusion It shows the higher pathological grade of glioma is caused by the rising of the Ki-67 and Bcl-2 expression levels,as well as the decreasing of Bax and Caspase3 expression levels.Meanwhile,it is very important in certain degree for the patients prognostic evaluation according to the judge the degree of glioma based on the Ki-67,Bcl-2,Bax and Caspase-3 expression levels.%目的 探讨脑胶质瘤患者瘤组织中Ki-67、Bcl-2、Bax和Caspase-3的表达水平及临床意义.方法 选取2014年10月～2016年10月武警后勤学院附属医院手术切除的65例脑胶质瘤组织切片.根据神经系统肿瘤分类将其分为低级别胶质瘤组34例和高级别胶质瘤组31例,另取18例正常脑组织切片标本作为对照组,比较3组标本组织中Ki-67、Bcl-2、Bax和Caspase-3表达水平,并分析其与胶质瘤恶性程度的相关性.结果 随着胶质瘤病理级别的升高,Ki-67表达水平逐渐增强,在正常脑组织、低、高级别胶质瘤组中的表达阳性率分别为0、20.6％、77.4％,差异有统计学意义(P＜0.05),二者呈正相关性(Spearman相关系数为0.645,P＜0.05).Bcl-2表达水平随着胶质瘤级别的升高而逐渐升高(6.2％、73.5％、100.0％),差异有统计学意义(P＜0.05),二者呈正相关性(Spearman相关系数为0.711,P＜0.05).Bax表达水平随着脑胶质瘤级别的升高而逐渐降低(100.0％、82.4％、l6.1％),差异有统计学意义(P＜0.05),二者呈负相关性(Spearman相关系数为-0.706,P＜0.05).Caspase-3表达水平在低级别胶质瘤组最高(0、100.0％、80.6％),随病理级别的升高而逐渐降低(p＜0.05),二者呈负相关性(Spearman相关系数为-0.334,P＜0.05).结论 Ki-67与Bcl-2表达水平的上调,Bax与Caspase-3表达水平的下调,提示胶质瘤病理级别越高.同时,根据Ki-67、Bcl-2、Bax和Caspase-3的表达水平可对胶质瘤恶性程度进行判断,在一定程度上对患者预后评估具有重要指导意义.