全自动酶标仪检测严重程度不同的急性痛风患者的IL-1β、TNF-α、COX-2动态变化情况与结果分析

摘要

Objective:To analyze dynamic change and severity of IL-1 beta, TNF alpha, COX-2 detected by automatic enzyme immunoassay analyzer on patients with acute gout.Methods: Ninety cases of acute gout patients selected according to the different degrees of severity, and were divided into three observed groups. Thirty health people were treated as the control group. IL-1β, TNF-α, COX-2 levels of dynamic change were recorded and compared within the four groups of patients.Results: The serum IL-1β, TNF-α, COX-2 levels in the 1st, 3rd, 7th, 10th day in the observation group 1 and group 2 in acute gout attacks were significantly higher than the control group; The serum levels of IL-1β, TNF-α, COX-2 levels in the 7th 10th day in observation group 1 in acute gout was significantly higher than observation group 2; The serum IL-1β, TNF-α,COX-2 levels in observation group 3 have not significant difference with control group 2; The serum IL-1β, TNF-α, COX-2 levels in observation group1,2,3 in acute gout attacks in the 14th days have not significant difference with control group.Conclusion: After analgesic drugs for the treatment of acute gout patients from 10 to 14 days, the inflammatory cytokines can be completely back to normal. The results provided important evidence for the introduction of analgesic drugs in patients with acute gout treatment.%目的：分析全自动酶标仪检测经镇痛药物治疗后严重程度不同的急性痛风患者的血清白细胞介素(IL-1β)、肿瘤坏死因子(TNF-α)及环氧合酶-2(COX-2)动态变化情况与结果。方法：前瞻性地选取90例急性痛风患者，按照痛风轻重程度的不同将其分为重度组、轻度组和缓解期组，每组30例。另选择30名体检健康者为对照组。使用镇痛药物秋水仙碱联合依托考昔对急性痛风患者进行治疗，采用ELISA法检测3组的血清IL-1β、TNF-α及COX-2水平动态变化情况。结果：重度组在急性痛风发作第1d、3d、7d和10d时的血清IL-1β水平与对照组相比明显升高，差异有统计学意义(t＝4.89，t＝5.26，t＝8.15，t＝3.74；P＜0.05)；重度组在急性痛风发作第7 d、10 d时的血清IL-1β水平与轻度组相比明显升高，差异有统计学意义(t=6.4931， t=3.3192；P＜0.05)；重度组在急性痛风发作的第1 d、3 d、7 d和10 d时的血清TNF-α水平与对照组相比明显升高，差异有统计学意义(t=3.24，t=3.47，t=3.60，t=4.22；P＜0.05)；重度组在急性痛风发作第10 d时的血清TNF-α水平与轻度组比较明显升高，差异有统计学意义(t=3.46， P＜0.05)；重度组在急性痛风发作的第1 d、3 d及7 d时的血清COX-2水平与对照组相比明显升高，差异有统计学意义(t=3.73，t=3.96，t＝8.27，t=4.22；P＜0.05)；重度组在急性痛风发作第7 d时的血清COX-2水平与轻度组相比明显升高，差异有统计学意义(t=6.3587，P＜0.05)；缓解期患者在急性痛风发作的10d血清IL-1β、TNF-α、COX-2的水平动态水平与对照组比较，差异无统计学意义(t=0.84，t=1.18，t=1.45；P＞0.05)。结论：使用镇痛药物对急性痛风患者进行治疗后，患者的IL-1β、TNF-α及COX-2等炎性细胞因子水平呈现动态变化，该研究为采用镇痛药物治疗急性痛风患者的疗程提供了重要的依据。