鹿丹通督片对兔脊髓压迫模型神经递质的影响

摘要

目的探讨鹿丹通督片对兔脊髓压迫模型脊髓乙酰胆碱酯酶(AChE)及P物质(SP)的影响。方法 16只家兔被复制成脊髓压迫模型，随机分为治疗组和对照组各8只。治疗组给服鹿丹通督片，对照组不用药，另选2只未造模、不用药家兔为正常组。脊髓AChE采用组织化学方法，SP采用免疫组织化学方法进行定性、定位观察，并应用扫描显微分光光度仪进行定量分析。结果脊髓AChE酶活性及SP免疫活性在造模后第7天，治疗组和对照组均比正常组减弱，治疗组活性明显高于对照组；扫描显微分光光度仪测定其OD值，两组间差异极其显著(均为P＜0.001)。结论鹿丹通督片对兔脊髓压迫模型的脊髓组织中的神经递质AChE和SP具有保护作用。%Objective To investigate the effect of Ludan Tongdu(LDTD)tablet on neurotransmitters of experimental spinal cord compression(SCC)in rabbits.Methods Spine cord compression models were made in 16 rabbits which were then divided into 2 groups randomly.The test-group was treated with LDTD tablet and the control-group without any drugs.2 other rabbits served as normal group i.e.without surgery and without adminastration of drugs.The acetyleholinesterase(AChE) and substance P(SP) in spinal cord were individually demonstrated with histochemical and immunohistochemical techniques.The quantitative analysis were carried out by computer scanning microspectrophotometer.Results 7 days after experiment,SCC AChE activity and SP immune activity in test-group and control-group were all lower than those in normal group,but those in test-group were higher than in control-group(P＜0.001).The OD of the two groups taken by computer scanning microspectrophotometer showed significantly difference (P＜0.001).Conclusion This study indicates that LDTD tablet could protect AChE and SP in the spinal cord of SCC.