AMPK、NF-κB、COX-2及PGE2在非小细胞肺癌中的表达

摘要

Objective To discuss the expressions and relations of AMP-activated protein kinase (AMPK),nuclear factor-κB (NF-κB),cyclooxygenase 2 (COX-2) and prostaglandin E2 (PGE2) in non-small cell lung cancer (NSCLC).Methods The expressions of phosphorylation product of acetyl coenzyme A carboxylase (PACC,which could directly reflect active AMPK),NF-κB and COX-2 in NSCLC and normal lung tissue specimens were detected by immunohistochemical SP method.The expressions of AMPK,COX-2 and EP2 in NSCLC,normal lung tissues and blood specimens were detected by qRT-PCR and Western blot.The SPSS 22.0 software package was used for statistical analysis.Results The positive expression rate of P-ACC in the NSCLC specimens was 33.9％ which was significantly lower than that in the normal lung tissue;while the positive expression rates of NF-KB and COX-2 in the NSCLC specimens were 75.8％ and 66.1％ respectively,which were significantly higher than those in the normal lung tissue.The expression of AMPK mRNA was reduced,while the expressions of COX-2 mRNA and PGE2 mRNA increased in the NSCLC specimens and blood,there were significant differences.Conclusions Low-expression of AMPK and high-expressions of NF-κB,COX-2 and EP2 in NSCLC may play important roles in NSCLC proliferation,apoptosis tolerance,invasion and metastasis.These different expressions provide a new idea for clinical treatment of NSCLC.%目的 探讨腺苷酸活化蛋白激酶(AMPK)、核因子κ B(NF-κ B)、环氧合酶2(COX-2)及前列腺素E2(PGE2)在非小细胞肺癌(NSCLC)中的表达及其关系.方法 采用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶连接法检测NSCLC和正常肺组织标本中可以直接反映AMPK激活状态的乙酰辅酶A羧化酶磷酸化后的产物(P-ACC)、NF-κB、COX-2的表达,实时荧光定量聚合酶链反应(qRT-PCR)和Western blot检测NSCLC标本及血液、正常肺组织标本及血液中AMPK、COX-2及PGE2受体的表达.结果 免疫组织化学法检测发现,P-ACC在NSCLC中低表达,NF-κB和COX-2在NSCLC中高表达,其阳性率分别为33.9％、75.8％和66.1％,且与正常肺组织中的表达差异有统计学意义;qRT-PCR和Western blot检测发现,在NSCLC标本及血液中,AMPK mRNA表达降低,COX-2和PGE2受体mRNA表达升高.结论 在NSCLC中,AMPK低表达,NF-κB、COX-2及PGE2高表达,其相互作用可能在NSCLC的增殖、凋亡耐受、侵袭及转移过程中占据重要位置,其表达情况为临床治疗NSCLC提供新的思路.