Objective To investigate the relationship between MMP9 and COX2 gene polymorphisms and chronic obstructive pulmonary disease. Methods A total of 71 patients with chronic obstructive pulmonary disease (COPD group) and 63 cases of acute respiratory infection and healthy persons as the objects of study. PCR-RFLP method was used to determine the polymorphisms of MMP9 (R279Q) and COX2 (1195G>A). The relationships between different genotypes and chronic obstructive pulmonary disease (COPD) were analyzed. Results Differences in MMP9 gene R279Q and GG and AA genotypes of COX2 (1195G>A) between the NC group and the COPD group had statistical significance (P = 0.010 and 0.021), the differences in the frequency of G and A alleles had statistical significance (P = 0.000). The G allele frequency at MMP9 R279Q locus (O^R = 2.24, 95％ CI: 1.59-7.03, P = 0.000) and the A allele frequency of COX2 gene 1195G>A locus (O^R = 2.12, 95％ CI: 1.57-6.82, P = 0.024) were the risk factors for COPD. MMP9 R279Q and COX21195G>A polymorphisms had correlations with FEV1％ pred, FEV1/FVC, MMP-9 and COX-2 (P = 0.015, 0.018, 0.009, 0.009, 0.021, 0.017, 0.015 and 0.000 respectively). Conclusions MMP9 gene R279Q and COX2 gene 1195G>A polymorphisms are the independent risk factors for COPD, and correlated with decrease of FEV1％pred and FEV1/FVC and increase of MMP-9 and COX-2.%目的 探讨MMP-9和COX-2基因多态性与慢性阻塞性肺疾病的关系.方法 选取71例慢性阻塞性肺疾病患者(COPD组)和同期63例急性呼吸道感染和健康体检者(NC组)作为研究对象,PCR-RFLP法测定MMP-9(R279Q)、COX-2(1195G>A)多态性,分析不同基因型与COPD的关系.结果 NC组与COPD组MMP-9(R279Q)基因、COX-2(1195G>A)的GG、AA基因型差异有统计学意义(P=0.010和0.021),G、A等位基因频率差异有统计学意义(P=0.000).MMP基因R279Q位点的G等位基因频率[O^R=2.24(95％CI:1.59,7.03)P=0.000],COX-2基因1195G>A位点的A等位基因频率[O^R=2.12(95％CI:1.57,6.82)P=0.024]为COPD的危险因素.MMP-9(R279Q)、COX-2(1195G>A)基因多态性与FEV1％pred、FEV1/FVC、MMP-9及COX-2具有相关性(P=0.015、0.018、0.009、0.009,0.021、0.017、0.015和0.000).结论 MMP-9基因R279Q位点、COX-2基因1195G>A位点多态性为COPD的危险因素,与FEV1％pred、FEV1/FVC下降及MMP-9、COX-2升高相关.
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